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Application of Calponin-h2 (CNN2) as marker for diagnosing ectopic pregnancy

A technology for ectopic pregnancy and diagnostic reagents, applied in disease diagnosis, microbial determination/examination, biological testing, etc., can solve the problem of difficult differential diagnosis of spontaneous abortion and ectopic pregnancy, high false positive and false negative rates, ectopic pregnancy Bleeding, etc.

Active Publication Date: 2019-07-05
张佳荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Continuous monitoring of blood β-hCG in the diagnosis of ectopic pregnancy has high false positive and false negative rates, and it is difficult to differentiate spontaneous abortion and ectopic pregnancy; at the same time, patients have ruptured ectopic pregnancy during multiple blood β-hCG monitoring risk of bleeding

Method used

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  • Application of Calponin-h2 (CNN2) as marker for diagnosing ectopic pregnancy
  • Application of Calponin-h2 (CNN2) as marker for diagnosing ectopic pregnancy
  • Application of Calponin-h2 (CNN2) as marker for diagnosing ectopic pregnancy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1, the expression of CNN2 in the villous tissue of ectopic pregnancy

[0078] The present inventors obtained intrauterine pregnancy villi tissues and ectopic pregnancy villi tissues of clinical experiment subjects, and analyzed and measured gene expression differences. Among them, there were 10 subjects of intrauterine pregnancy and 10 subjects of ectopic pregnancy.

[0079] The determination adopts a conventional method of obtaining tissue, extracting nucleic acid, and performing PCR detection.

[0080] The PCR primers used are as follows:

[0081] Forward: 5'-ATGAACAAGCTACAGCCGGG-3' (SEQ ID NO: 1);

[0082] Reverse: 5'-CTTAGTCTTGGCCTGCGTCA-3' (SEQ ID NO: 2);

[0083] The measurement results of the expression of CNN2 are as follows: figure 1 As shown, the expression of CNN2 in ectopic pregnancy villi was significantly higher than that in intrauterine pregnancy villi.

Embodiment 2

[0084] Example 2, the expression of CNN2 in the fallopian tube of ectopic pregnancy blastocyst implantation

[0085] The inventors obtained the fallopian tube tissue of the blastocyst implantation part and the part of the fallopian tube tissue far away from the blastocyst implantation of patients with clinical ectopic pregnancy, and analyzed and measured the differences in protein expression of CNN1, CNN2, and CNN3. Among them, there were 10 ectopic pregnancy subjects.

[0086] Determination adopts conventional immunohistochemical staining and ELISA detection, respectively, with anti-CNN1 antibody (mAb, purchased from Proteintech Group, Inc., Chicago, IL, USA), anti-CNN2 antibody (mAb, purchased from Novus Biologicals, CO, USA), anti-CNN3 antibody (monoclonal antibody, purchased from Proteintech Group, Inc., Chicago, IL, USA) for detection.

[0087] The results of immunohistochemistry were as figure 2 , CNN2 was highly expressed in the fallopian tube where the blastocyst im...

Embodiment 3

[0089] Example 3, the expression of CNN2 in serum

[0090] The inventor obtained 84 patients with ectopic pregnancy, 39 women with intrauterine pregnancy, 9 cases of missed abortion, and 32 non-pregnant controls. The blood of these study subjects was drawn to obtain serum. The expression of CNN2 in serum was determined by ELISA port visual inspection.

[0091] The results of ELISA are shown in Table 2, which indicated that CNN2 was highly expressed in the serum of patients with ectopic pregnancy.

[0092] Table 2

[0093]

[0094]

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Abstract

The invention relates to an application of Calponin-h2 (CNN2) as a marker for diagnosing ectopic pregnancy. The invention discloses for the first time that CNN2 can be used as a specific serum markerfor early diagnosis of ectopic pregnancy, and has good sensitivity and specificity. The application of CNN2 in the auxiliary diagnosis of ectopic pregnancy can promote the timeliness and accuracy of diagnosis and effectively improve the early diagnosis rate of ectopic pregnancy.

Description

technical field [0001] The invention belongs to the field of diagnostic markers, more specifically, the invention relates to the early diagnosis of ectopic pregnancy, and the application of Calponin-h2 as a marker for diagnosing ectopic pregnancy. Background technique [0002] Ectopic pregnancy refers to the implantation of a fertilized egg outside the uterine body cavity, and is one of the most common acute abdominal diseases in obstetrics and gynecology. Ruptured tubal pregnancy can cause intra-abdominal hemorrhage, infection, shock and other complications, and severe cases can endanger the life of the patient. Ectopic pregnancy is one of the most common acute abdomen in obstetrics and gynecology, which can lead to massive intra-abdominal hemorrhage, shock and even death of patients. In developed countries, 9-13% of maternal deaths are caused by ectopic pregnancy, while in developing countries, this proportion is as high as 10-30%. [0003] At present, the early diagnosi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G01N33/68
CPCC12Q1/6883G01N33/689C12Q2600/158G01N2800/368
Inventor 张佳荣李双弟
Owner 张佳荣
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