Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Gene enhancing herbicide resistance of rubber plants, mediating vector and application of gene

A herbicide-resistant gene and rubber-enhancing technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of less molecular biology research, unable to meet industrial needs, and difficult to attract attention.

Inactive Publication Date: 2019-06-21
肖宇 +1
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Rubber is an important industrial raw material and strategic material. Although rubber grass is a natural rubber-producing plant, its output cannot be compared with that of the Brazilian rubber tree, and it cannot meet the industrial needs, so it is difficult to attract attention. , has caused the Soviet Union, the United States, China, etc. to conduct research on its morphological structure, cultivation techniques, and tissue physiology, but there are few studies on molecular biology.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene enhancing herbicide resistance of rubber plants, mediating vector and application of gene
  • Gene enhancing herbicide resistance of rubber plants, mediating vector and application of gene
  • Gene enhancing herbicide resistance of rubber plants, mediating vector and application of gene

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0010] Embodiment 1: The nucleotide sequence of the herbicide-resistant gene of the enhanced rubber grass in this embodiment is shown in SEQ ID NO:1.

[0011] The extraction method of the gene of the present embodiment is as follows:

[0012] 1. Enhance the extraction of the total DNA of the rubber grass herbicide resistance gene:

[0013] (1) Take 5-20mL of CTAB extraction buffer and preheat it at 68°C. CTAB extraction buffer: 2% CTAB, 100mmol / L Tris-Cl, 20mmol / LEDTA, 1.4mol / L NaCl, 2% sulfhydryl by volume Ethanol, pH8.0;

[0014] (2) Take 0.5 g of Agrobacterium Rhizogenes (Agrobacterium Rhizogenes, R1601) and grind it into powder with liquid nitrogen, grind it as finely as possible, transfer it to an Eppendorf tube, add 700 μL of preheated CTAB extraction buffer, shake vigorously and mix well;

[0015] (3) Add 50 μL of 20% SDS, 50 μL of 10% PVP and 20 μL of mercaptoethanol, mix gently by inversion, and place in a 68°C water bath for 30 minutes;

[0016] (4) Add 300μL CI t...

specific Embodiment approach 2

[0035] Specific embodiment two: This embodiment contains the mediation carrier of the enhanced rubber grass resistance herbicide gene of the specific embodiment one.

[0036] The mediating carrier of this embodiment is Agrobacterium LBA4404.

specific Embodiment approach 3

[0037] Embodiment 3: The gene for enhancing rubber grass resistance to herbicides as described in Embodiment 1 is used for genetic improvement of rubber grass.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a gene enhancing herbicide resistance of rubber plants, a mediating vector containing the gene enhancing the herbicide resistance of the rubber plants and an application of thegene in genetic improvement of the rubber plants. The nucleotide sequence of the gene is shown in SEQ ID NO:1. The gene enhancing the herbicide resistance of the rubber plants is applied to genetic transformation of the rubber plants, and the herbicide resistance of the rubber plants is improved after transformation.

Description

technical field [0001] The invention relates to a gene, a mediating carrier and applications. Background technique [0002] Rubber is an important industrial raw material and strategic material. Although rubber grass is a natural rubber-producing plant, its output cannot be compared with that of the Brazilian rubber tree, and it cannot meet the industrial needs, so it is difficult to attract attention. , has caused the Soviet Union, the United States, China, etc. to conduct research on its morphological structure, cultivation techniques, and tissue physiology, but there are fewer studies on molecular biology. Contents of the invention [0003] The invention provides a gene, a mediating carrier and application for enhancing the resistance of rubber grass to herbicides. [0004] The nucleotide sequence of the herbicide-resistant gene of the enhanced rubber grass of the present invention is shown in SEQ ID NO:1. [0005] The mediation carrier containing the above-mentioned ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/31C12N15/82A01H5/00
Inventor 肖宇曾祥俊孙力
Owner 肖宇
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com