Preparation method of mouse chronic atrophic gastritis model

Abstract

The invention relates to a preparation method of a mouse chronic atrophic gastritis model, which comprises the following steps: filling N-methyl-N-nitro-nitrosoguanidine according to the weights of the mice, using low-content ammonia water as feeding and drinking water; after the intervention by using the N-methyl-N-nitro-nitrosoguanidine, filling helicobacter pylori with positive CagA and VagA; wherein the mice are fasted before gastric administration and fasted and fasted with water after gastric administration; extracting male and female mice, carrying out gastric mucosa histopathological examination, and isolating and culturing the helicobacter pylori from the gastric mucosa to confirm the establishment of the helicobacter pylori and the formation of inflammation. The method uses miceto construct chronic atrophic gastritis, has simple operation, low cost, short period, high success rate, stable pathological change and strong repeatability, has outstanding substantive advantages, and provides a good animal model for studying chronic atrophic gastritis, gastric cancer and other diseases caused by helicobacter pylori infection.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a preparation method of a mouse chronic atrophic gastritis model. Background technique [0002] Chronic atrophic gastritis (CAG) is a precancerous disease of the stomach. Its onset is slow, lingering, protracted, and difficult to treat. If it is not well controlled, it may develop into intestinal metaplasia or atypical hyperplasia, and finally form stomach cancer. The research and treatment of CAG have attracted much attention. The modeling of CAG animal models is the key material for the basic and clinical research of the disease. A good animal model will provide direct in vivo evidence for the prevention and treatment of the disease. The main methods for constructing animal models of chronic atrophic gastritis include chemical carcinogenesis and Helicobacter pylori infection, etc., but so far, the construction of the model is not ideal, and there are still many areas worth ...

AI Technical Summary

Problems solved by technology

The main methods for constructing animal models of chronic atrophic gastritis include chemical carcinogenesis and Helicobacter pylori infection, etc., but so far, the construction of the model is not ideal, and there are still many areas worth exploring, such as the selection of experimental animals, the combination of drugs and The size of the dose, the length of modeling time, the ease of operation, etc.

N-methyl-N-nitro-nitrosoguanidine, ammonia water, sodium deoxycholate, sodium salicylate, etc. are currently commonly used chemical modeling materials, and rats or Mongolian gerbils are basically used for modeling , mice are rarely used to establish models of chronic atrophic gastritis, the reason may be that mice are not easy to control the dose of intervention, and are prone to other complications or side effects; Helicobacter pylori is the main cause of gastritis and gastric ulcer, It is the only pathogenic bacterium for constructing CAG animals. The animal model it constructs is closer to the CAG caused by human infection, which is more conducive to the study of the pathogenesis of CAG. However, Helicobacter pylori is easier to colonize in rats and Mongolian gerbils, and the incidence is relatively high , but it is difficult to colonize in mice. The clinically isolated strains can only be colonized after adaptive domestication, and the colonization period is relatively short. Generally, the colonization amount reaches the maximum in the first month after infection, and the colonization amount will decrease in the second and third months. decreased, and could not maintain a relatively high infection rate; Zeng Zhirong, the First Affiliated Hospital of Sun Yat-sen Medical University, infected C57BL / 6 mice and BALB / c mice with the Sydney strain of Helicobacter pylori (SS1), although the strain maintained in the body for a long time High colonization rate, but the strain is CagA-negative, so that chronic atrophic gastritis has not yet appeared 24 weeks after the last inoculation; Wang Jian, Zhongshan Hospital Affiliated to Fudan University, et al. Nitrosourea (MNU)" joint modeling, chronic atrophic gastritis in mice at 36 weeks is 23.1%, which requires a long period and the success rate is low; we used mice in the preliminary experiment, using "Helicobacter pylori group +N-Methyl-N-nitro-nitrosoguanidine group" method, the mice did not appear chronic atrophic gastritis in the 4th month after the last infection

It can be seen that it is very difficult to prepare chronic atrophic gastritis in mice infected with Helicobacter pylori in a short period of time.

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