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Method for analyzing corn borer population genetic diversity by ISSR system

A genetic diversity and corn borer technology, applied in the field of molecular biology, can solve the problem of less research on the genetic diversity of corn borer populations, and achieve the effects of high polymorphism, stable PCR amplification reaction, and clear amplification fragments

Active Publication Date: 2019-04-05
黑龙江省植检植保站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the analysis of genetic diversity within and between species of insects using ISSR molecular markers.

Method used

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  • Method for analyzing corn borer population genetic diversity by ISSR system
  • Method for analyzing corn borer population genetic diversity by ISSR system
  • Method for analyzing corn borer population genetic diversity by ISSR system

Examples

Experimental program
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specific Embodiment approach 1

[0040] Specific embodiment one: a kind of ISSR molecular marker primer that is used for the genetic diversity analysis of corn borer population of the present embodiment, described primer sequence comprises:

[0041] Primer 807: its nucleotide sequence is shown in the sequence table Seq ID No: 1;

[0042] Primer 810: its nucleotide sequence is shown in the sequence table Seq ID No: 2;

[0043] Primer 818: its nucleotide sequence is shown in the sequence table Seq ID No: 3;

[0044] Primer 825: its nucleotide sequence is shown in the sequence table Seq ID No: 4;

[0045] Primer 826: its nucleotide sequence is shown in the sequence table Seq ID No: 5;

[0046] Primer 835: its nucleotide sequence is shown in the sequence table Seq ID No: 6;

[0047] Primer 836: its nucleotide sequence is shown in the sequence table Seq ID No: 7;

[0048]Primer 847: its nucleotide sequence is shown in the sequence table Seq ID No: 8;

[0049] Primer 848: its nucleotide sequence is shown in th...

specific Embodiment approach 2

[0055] Embodiment 2: An ISSR-PCR reaction system for analyzing the genetic diversity of corn borer populations in this embodiment, each 25 μL reaction system contains 1 μL of template, 0.125 μL of Taq enzyme, 2.5 μL of Buffer, 2 μL of dNTP, and 1 μL of primer , ddH 2 O18.375 μL; Amplify the above reaction system according to the following procedure: pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 45 seconds, annealing at 40-61°C for 1.5 minutes, extension at 72°C for 1.5 minutes, a total of 40 cycles, and finally extension at 72°C for 10 minutes , stored at 4°C.

specific Embodiment approach 3

[0056] Embodiment 3: This embodiment is different from Embodiment 2 in that the primer is selected from any one of Seq ID Nos: 1-14 described in Embodiment 1.

[0057] Others are the same as in the second embodiment.

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Abstract

The invention provides a method for analyzing the corn borer population genetic diversity by an ISSR system, and relates to a method for analyzing the corn borer population genetic diversity. The invention aims at solving the problems that in the prior art, no effective molecular marking method, particularly an ISSR molecular marking method is used for identifying the corn borer, particularly thegenetic structure and the affinity relationship of different geographical populations of asiatic corn borer, so that the migration change condition of the corn borer is specified. The invention provides the method for analyzing the corn borer population genetic diversity by the ISSR system. The method is a simple and easy-to-operate molecular marking technology; for 14 ISSR primer sequences selected by the method, the PCR amplification reaction in the corn borer is stable; the amplified fragments are clear; the polymorphism is high; the technical support is provided for scientific research such as corn borer resource identification and genetic diversity analysis. The method is applied to the field of the corn borer.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a method for analyzing the genetic diversity of corn borer populations by using an ISSR system. Background technique [0002] The vast majority of species in nature will have non-random gene flow due to their differences in behavior and asymmetric environmental factors, resulting in different degrees of genetic differences among species populations in different regions. Due to the phenomenon of differentiation and formation of new species among different populations, the diversity of population genetic structure and population genetics also arise. [0003] Molecular markers are one of the important technologies for studying population genetics at present. With the rapid development of molecular biology research techniques, various molecular marker technologies have emerged. ISSR molecular markers are derived from SSR molecular markers. With the advantages of SSR mole...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 王振宋显东杨峰山张齐凤李鹏司兆胜张丙芳
Owner 黑龙江省植检植保站
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