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Tissue culture method for quickly propagating big cherries

A cherry and fast technology, applied in the field of tissue culture for rapid propagation of large cherries, can solve problems such as slow growth, and achieve the effects of promoting growth, improving the efficiency of proliferation and culture, and improving the survival rate.

Inactive Publication Date: 2019-03-08
南京大花滩生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this invention has the above advantages, there are still problems such as slow growth in the tissue culture process of large cherry, therefore, it is necessary to improve the program

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for tissue culture of large cherry rapid propagation, comprising the following steps:

[0025] (1) get the required cherry rootstock sapling terminal bud, inoculate and cultivate on the induction medium after disinfection;

[0026] (2) Get the shoot tip of the growing bud cluster in the step (1), inoculate it on the proliferation medium and cultivate it;

[0027] (3) the seedlings in the step (2) are cut off, inoculated and cultivated in the rooting medium;

[0028] (4) When the seedlings are cultivated on the rooting medium for 10 days and the root length reaches 1 cm, take out the seedlings and wash off the root medium, move them to the nutrient soil, spray through them with 0.1% carbendazim, and place them in a plastic shed , Spray the nutrient solution every 2 days. When the leaves start to grow, move them out of the plastic shed after seven days, ventilate, increase the light, and then move them into the field.

[0029] Specifically, the formulation of t...

Embodiment 2

[0035] A method for tissue culture of large cherry rapid propagation, comprising the following steps:

[0036] (1) get the required cherry rootstock sapling terminal bud, inoculate and cultivate on the induction medium after disinfection;

[0037] (2) Get the shoot tip of the growing bud cluster in the step (1), inoculate it on the proliferation medium and cultivate it;

[0038] (3) the seedlings in the step (2) are cut off, inoculated and cultivated in the rooting medium;

[0039] (4) When the seedlings are cultivated on the rooting medium for 10 days and the root length reaches 1 cm, take out the seedlings and wash off the root medium, move them to the nutrient soil, spray through them with 0.1% carbendazim, and place them in a plastic shed , Spray the nutrient solution every 2 days. When the leaves start to grow, move them out of the plastic shed after seven days, ventilate, increase the light, and then move them into the field.

[0040]Specifically, the formulation of th...

Embodiment 3

[0046] A method for tissue culture of large cherry rapid propagation, comprising the following steps:

[0047] (1) get the required cherry rootstock sapling terminal bud, inoculate and cultivate on the induction medium after disinfection;

[0048] (2) Get the shoot tip of the growing bud cluster in the step (1), inoculate it on the proliferation medium and cultivate it;

[0049] (3) the seedlings in the step (2) are cut off, inoculated and cultivated in the rooting medium;

[0050] (4) When the seedlings are cultivated on the rooting medium for 10 days and the root length reaches 1 cm, take out the seedlings and wash off the root medium, move them to the nutrient soil, spray through them with 0.1% carbendazim, and place them in a plastic shed , Spray the nutrient solution every 2 days. When the leaves start to grow, move them out of the plastic shed after seven days, ventilate, increase the light, and then move them into the field.

[0051] Specifically, the formulation of t...

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PUM

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Abstract

The invention belongs to the field of a tissue culture method for big cherries, and specifically relates to the tissue culture method for quickly propagating the big cherries. The tissue culture method comprises the following steps: (1) taking terminal buds of required cherry rootstock treelets, and inoculating to an induction culture medium for culturing after sterilizing; (2) taking stem tips ofclumpy buds growing in step (1), and inoculating to a proliferation culture medium for culturing; (3) cutting down seedlings in step (2), and inoculating to a rooting culture medium for culturing; (4) taking out plantlets and washing out the rooting culture medium when the plantlets are cultured on the rooting culture medium for 1o days and the root length is up to 1 to 1.5 cm, transplanting in nutritional soil, completely spraying by using 0.1 percent of carbendazim, putting in a plastic shed, spraying a nutritional solution for once every two days, transplanting out of the plastic shed after seven days when blades start to grow, ventilating, increasing illumination, and transplanting in a field. According to the big cherries cultured and obtained through the tissue culture method disclosed by the invention, not only can the big cherries grow quickly, but also the survival rate of the big cherries can be increased; in addition, the edible taste of the big cherries can also be increased by sugar in the nutritional solution.

Description

technical field [0001] The invention belongs to the field of tissue culture methods of large cherries, and in particular relates to a tissue culture method for rapid propagation of large cherries. Background technique [0002] Cherry is a kind of plant of Rosaceae and Prunus, the earliest fruit tree species of deciduous fruit trees in northern China after Chinese cherry. According to traditional Chinese medicine, cherries have the functions of regulating qi and dispelling rheumatism. [0003] The tissue culture technique of higher plants refers to the technique of isolating one or several somatic cells or a part of the plant body for culture. Usually, what we call tissue culture in a broad sense refers to separating a part of a plant body through aseptic operation, inoculating it on a medium, and cultivating it under artificially controlled conditions to produce a complete plant. Tissue culture can be divided into tissue or callus culture, organ culture, plant culture, cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 史月龙魏浩钧傅琦俊曾宋君吴坤林吴英亮郑枫汤静
Owner 南京大花滩生物科技有限公司
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