Method for simultaneously constructing experimental models of cryptococcal pneumonia and meningitis

Abstract

The invention relates to a method for constructing an experimental model, specifically to a method for constructing experimental models of cryptococcal pneumonia and meningitis, particularly to a method for simultaneously constructing experimental animal models of cryptococcal pneumonia and meningitis, belonging to the field of biomedicine and experimental technology. According to the invention, an otoendoscope is adopted to optimize the determination of the intervention manner of Cryptococcus liquid, so experimental mouse models of pneumonia and meningitis are simultaneously constructed. Results show that the experimental mouse meningitis model constructed by using the method of the invention can better simulate the clinical natural infection process of Cryptococcus; i.e., the respiratorytract is infected by Cryptococcus at first, and then Cryptococcus is disseminated to all parts of the body, which leads to cryptococcal meningitis; the method is simple and feasible and has good repeatability; the models have small damage to experimental mice and allow the amount of cryptococcal infection to be relatively constant; and good experimental animal models are provided for further research on the pathogenicity and drug resistance of Cryptococcus and for screening of novel drugs.

Description

technical field [0001] The invention belongs to the field of biomedicine and experiment technology, and relates to a method for constructing an experimental model, in particular to a method for constructing an experimental model of cryptococcal pneumonia and meningitis, in particular to a method for simultaneously constructing experimental animal models of pulmonary cryptococcal pneumonia and meningitis method. Background technique [0002] The prior art discloses that Cryptococcus neoformans (Cryptococcus neoformans) has another name called Torula Histolytica (Torula Histolytica), which is a saprophytic bacterium of soil, pigeons, milk, fruits, etc. Germs. Studies have shown that most of the pathogenic bacteria enter the human body through the respiratory tract, causing mild inflammation in the lungs, and most of them are hidden infections. Nervous system, causing diseases such as cryptococcal meningitis, encephalitis or brain granuloma. Clinical practice shows that the ...

AI Technical Summary

Problems solved by technology

In clinical practice, the establishment of mouse experimental models is often used to carry out the above-mentioned related research. At present, the method of intracerebroventricular injection is mainly used to construct the mouse cryptococcal meningitis model. Accidents that can easily lead to accidental death of mice. The more critical issue is that since the cryptococcus is directly injected into the ventricle of the mouse in this method, it is different from the clinical practice that most patients are infected with cryptococcus through the respiratory tract and spread through the bloodstream to cause cryptococcus. The natural infection route and process of meningococcus are completely different. Therefore, it is impossible to truly observe the immune state of the body in the disease and obtain valuable experimental evidence. The experimental model of the natural infection process and its construction method to meet the clinical research needs of the above-mentioned cryptococcus and its induced meningitis

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