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Application of baicalin in the preparation of drugs for preventing neural tube defects in fetuses with gestational diabetes mellitus

A technology for neural tube defects and diabetes, which is applied in the field of medicine, can solve ambiguity and other problems, and achieve the effect of improving self-renewal and reducing the level of retinoic acid

Active Publication Date: 2020-05-08
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, whether baicalin can prevent neural tube defects in fetuses with gestational diabetes is not yet clear

Method used

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  • Application of baicalin in the preparation of drugs for preventing neural tube defects in fetuses with gestational diabetes mellitus
  • Application of baicalin in the preparation of drugs for preventing neural tube defects in fetuses with gestational diabetes mellitus
  • Application of baicalin in the preparation of drugs for preventing neural tube defects in fetuses with gestational diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Different Concentrations of Baicalin Improve Chicken Embryo Neural Tube Defects and Embryo Mortality under High Sugar Environment

[0037] Based on the chicken embryo model of gestational diabetes we have established (Cell Cycle: 14(5):772-783.2015), we used the method of Early chick (EC) Culture to culture early chicken embryos, and divided the chicken embryos into the control group (0.1% DMSO ), high-sugar group (50mM glucose), high-sugar+3μM baicalin group, high-sugar+6μM baicalin group, and high-sugar+12μM baicalin group were totally 5 groups (n=30 embryos in each group; baicalin was dissolved with DMSO , the concentration of DMSO in the working solution of the experimental group used was lower than 0.1%). Photographs were taken at 0 hours, 24 hours and 36 hours of the experiment ( figure 1A-E2; 36-hour high-magnification images of the head (A3-E3). The embryos in the normal group had no death and neural tube defects, and there were 10.3±0.1528 pairs of ...

Embodiment 2

[0039] Example 2 Baicalin improves neural tube developmental abnormalities in day 1.5 embryos caused by high sugar environment

[0040] Based on the concentration study in Example 1, we divided chicken embryos into control group (0.1% DMSO), high sugar group (50mM glucose), baicalin group (6μM), glucose+baicalin group (50mM glucose+6μM baicalin) 4 Groups (n=20 embryos per group). After baicalin was dissolved in DMSO, the concentration of DMSO in the working solution of the experimental group used was lower than 0.1%. The reagents of each group above were added to EC Culture, and then the chicken embryos were cultured to the HH10 stage, and the embryos were subjected to PAX7 immunofluorescence staining, and the neural tube morphology of the HH10 chicken embryos was observed. There were no neural tube defects in the head and trunk of the embryos in the control group and the baicalin group ( figure 2 A-B1, E). The incidence rate of neural tube defects in the head and / or trunk...

Embodiment 3

[0042] Example 3 Baicalin improves neural tube developmental abnormalities in 4.5-day embryos caused by high-sugar environment

[0043] Chicken embryos were divided into control group (0.1% DMSO), high glucose group (50mM glucose), baicalin group (6μM), glucose+baicalin group (50mM glucose+6μM baicalin) 4 groups (n=15), We adopt the method of adding medicine to the air chamber (for the specific operation of adding medicine to the air chamber, refer to J Exp Biol.218 (Pt 21): 3468-77.2015; Cell Physiol Biochem.48 (5): 2084-2090.2018), and perform the above procedures on chicken embryos. Embryos were collected 4.5 days after treatment in groups of 4. from image 3 A-B1 We can observe that the embryos in the control group and the baicalin group are well developed, the shape is normal, the midbrain has been formed normally under high power microscope, and there is no neural tube defect; some embryos in the high glucose treatment group ( image 3 C-C1) A malformation of midbrain ...

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Abstract

The invention provides application of baicalin in preparation of a drug for treating a fetal neural tube defect caused by gestational diabetes mellitus. Based on a model of a fetus with gestational diabetes mellitus, which is established by a chick embryo, a chick embryo experiment is performed by adopting a traditional Chinese medicine monomer baicalin, a result shows that a certain concentrationof baicalin can be used for effectively relieving the neural tube defect induced by a high glucose environment and effectively relieving poor neural tube cell differentiation, elevation of the levelof homocysteine, reduction of the level of retinoic acid and inhibition on self-renewing of neural stem cells caused by the high glucose environment. According to the application, the application range of the baicalin is enlarged, the application value of the baicalin is improved, and the development of new drugs can be further facilitated, for example, the activity of the baicalin is hopefully improved, or the side effect of the baicalin is reduced by structural modification or transformation by taking the baicalin as a lead compound; the safety drug is provided for preventing the neural tubedefect of the fetus with gestational diabetes mellitus, therefore, the application has good application prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of baicalin in the preparation of medicines for fetal neural tube defects of gestational diabetes mellitus. Background technique [0002] Neural tube defect, also known as neural tube defect, is a serious deformity disease, including anencephaly, encephalocele, exocephalus, craniospina bifida, etc. The incidence of neural tube defects in my country is about 2.74‰. It is reported that the incidence of congenital malformations in fetuses with gestational diabetes is 2 to 5 times higher than that in non-diabetic pregnancies, mainly manifested as nervous system abnormalities and congenital heart diseases. We can use chicken embryos to establish a fetal model of gestational diabetes (Cell Cycle: 14(5):772-783.2015), which provides a good in vivo model for the present invention to study drugs that protect fetal development of gestational diabetes. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61P3/10A61P25/00
CPCA61K31/7048A61P3/10A61P25/00
Inventor 王广卢嘉琪杨雪松梁健欣司振鹏
Owner JINAN UNIVERSITY
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