A kind of Cymbidium rapid propagation seedling raising method

A fast, seedling technology, applied in the field of artificial planting of rare and endangered plants, can solve the problems of difficult germination, high cost and low reproduction rate

Active Publication Date: 2021-09-14
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the Cymbidium seed is small, has no endosperm, and is extremely difficult to germinate under natural conditions, and the traditional Cymbidium ramet propagation method has the disadvantages of low reproduction rate, slow propagation speed and high cost, and provides a method for using Cymbidium Seed Rapidly and Efficiently Propagating and Raising Seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Seed treatment

[0028] Select Cymbidium pods that are 18-24 weeks after pollination and fully developed, put them in a cowhide bag to dry in the shade, bury them in fine sand, and store them in a dry place at 15°C in a dark place for 8 weeks, and then put the Cymbidium pods Rinse with sterile water 3 to 5 times to remove surface debris, peel off the Cymbidium pods and pour out the seeds, disinfect the seeds with 75% ethanol aqueous solution under sterile conditions for 0.75min, and rinse with sterile water 3 to 5 times, then add mercuric chloride aqueous solution with a volume concentration of 0.15% for disinfection treatment for 7 minutes, rinse with sterile water for 3 to 5 times, and obtain the sterilized seeds; soak the sterilized seeds in 1.0mg / L red Mycin GA 3 The aqueous solution was placed in a shaker and oscillated at 180 r / min for 48 hours at 25°C.

[0029] 2. Sowing and cultivating

[0030] Under sterile conditions, mix the treated seeds in step 1 with...

Embodiment 2

[0045] 1. Seed treatment

[0046] Select Cymbidium pods that are 18-24 weeks after pollination and fully developed, put them in a cowhide bag to dry in the shade, bury them in fine sand, and store them in a dry place at 15°C in a dark place for 8 weeks, and then put the Cymbidium pods Rinse with sterile water 3 to 5 times to remove surface debris, peel off the Cymbidium pods and pour out the seeds, disinfect the seeds with 75% ethanol aqueous solution under sterile conditions for 0.75min, and rinse with sterile water 3 to 5 times, then add mercuric chloride aqueous solution with a volume concentration of 0.15% for disinfection treatment for 7 minutes, rinse with sterile water for 3 to 5 times, and obtain the sterilized seeds; soak the sterilized seeds in 0.5mg / L red Mycin GA 3 The aqueous solution was placed in a shaker and oscillated at 180 r / min for 48 hours at 25°C.

[0047] 2. Sowing and cultivating

[0048] Under sterile conditions, mix the treated seeds in step 1 with...

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PUM

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Abstract

The invention discloses a method for rapidly propagating and raising seedlings of Cymbidium. In the method, seeds of Cymbidium are sterilized with gibberellin GA 3 treatment, and then sow them in sterile germination medium for seedling cultivation under sterile conditions. After 2 to 3 true leaves grow and the root system is fully developed, they are transplanted into the transfer medium for transfer culture, and cultured for 2 to 3 Acclimate after one month, and finally transplant to grow in the transplanting medium. The method of the invention is simple and easy to implement, low in cost, high in seed germination rate, which can reach 30% to 50%, short germination period, high transplanting survival rate, which can reach more than 90%, and can quickly and massively obtain Cymbidium seedlings. Meet the needs of large-scale, large-scale seedling cultivation.

Description

technical field [0001] The invention belongs to the technical field of artificial planting of rare and endangered plants, and in particular relates to a rapid propagation and seedling raising method of Cymbidium orchidaceae. Background technique [0002] Cymbidium faberi (Cymbidium faberi) is a ground herbaceous plant of the genus Orchidaceae, with inconspicuous pseudobulbs. As a large species of Chinese orchids in the traditional sense, it is deeply loved by people for its rich and pure aroma, elegant and graceful flower shape, and elegant and firm plant shape. At present, it is mainly produced in southern Shaanxi, southern Gansu, Anhui, Zhejiang, Jiangxi, Fujian, Hubei, Hunan, Guizhou, Yunnan and other places. Cymbidium has a long history of cultivation, and there are many traditional fine varieties. Some of them have been cultivated for hundreds of years. [0003] Cymbidium grows naturally in hillside forests, and most of the cultivated varieties are selected from the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00A01G24/28A01G24/23A01G24/15
CPCA01G31/00A01H4/001A01H4/008A01G24/15A01G24/23A01G24/28
Inventor 王喆之刘帅牛俊峰王世强吴小强张淑柯胡喜风胡晓瑜黄飞
Owner SHAANXI NORMAL UNIV
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