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Compositions and methods for gene editing

A technology for targeting genes and complexes, which is applied in the field of compositions and methods for gene editing, and can solve problems such as being unsuitable for gene editing tools

Inactive Publication Date: 2019-01-04
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, TtAgo requires high temperature (>65°C) to achieve its endonuclease activity and thus is not suitable as a gene editing tool

Method used

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  • Compositions and methods for gene editing
  • Compositions and methods for gene editing
  • Compositions and methods for gene editing

Examples

Experimental program
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Effect test

Embodiment approach 1

[0313] Embodiment 1. In some embodiments, a method of modifying a target nucleic acid is provided, comprising contacting the target nucleic acid with an Argonaute (Ago) protein and a single-stranded guide DNA at a temperature of about 10° C. to about 60° C., wherein the The Ago protein and the guide DNA form a complex that specifically recognizes a target locus in the target nucleic acid, and wherein the target locus comprises a sequence complementary to that of the guide DNA.

Embodiment approach 2

[0314] Embodiment 2: In other embodiments according to embodiment 1, the Ago protein cleaves the target locus.

Embodiment approach 3

[0315] Embodiment 3: In still other embodiments according to embodiment 2, wherein the target locus is double stranded DNA, the Ago protein induces a double strand break at the target locus.

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Abstract

The present application provides methods, compositions, delivery systems, and kits for modifying a target nucleic acid using an Argonaute (Ago) and a single-stranded guide DNA. In some embodiments, methods of gene editing in a cell, such as a mammalian cell, are provided.

Description

[0001] Cross references to related patent applications [0002] This application claims the priority rights of Chinese Patent Application No. 201510971234.5 filed on December 21, 2015 and Chinese Patent Application No. 201610349444.5 filed on May 25, 2016, the contents of each patent application are incorporated herein by reference in their entirety middle. [0003] Submit an ASCII text file of the sequence listing [0004] The following submission as an ASCII text file is hereby incorporated by reference in its entirety: Computer Readable Form of the Sequence Listing (CRF) (File Name: 777072000140SEQLIST.txt, Date of Record: December 15, 2016, Size: 309KB). technical field [0005] The present invention relates to Argonaute-based methods, compositions and delivery systems useful for sequence-specific modification of target nucleic acids, including gene editing in cells. Background technique [0006] In-depth understanding of genetic information and the development of gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12N15/85C12N15/86C12N15/55C12N9/22C12N15/113
CPCC12N15/102C12N15/113C12N2310/20C07K14/195C12N15/86
Inventor 沈啸韩春雨
Owner ZHEJIANG UNIV
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