Preparing method for tetrodotoxin standard biological samples

Abstract

The invention discloses a preparing method for tetrodotoxin standard biological samples. The preparing method includes the steps that nassarius samples are extracted with an acetic-acid methanol solution, a crude extracting solution is purified with a carbon-nano-tube solid-phase extraction column, the purified venom is subjected to high-performance-liquid-chromatography purification, and then isfrozen, dried and concentrated, crystals are separated out, and the tetrodotoxin standard biological samples are obtained. According to the preparing method for the tetrodotoxin standard biological samples, in the TTX extraction aspect, the purifying aspect and the pure-product preparing aspect, the nassarius serves as a raw material, extraction is carried out with the acetic-acid methanol solution, the crude extracting solution is purified through the large-volume-carbon-nano-tube solid-phase extraction column, then the purified venom is concentrated, and the samples are purified and preparedthrough high-performance-liquid sample feeding. The sample preparing method and process are easy and convenient to operate, efficient and suitable for batch production and technology preparation of the tetrodotoxin standard biological samples.

Description

technical field [0001] The invention relates to a method for preparing a tetrodotoxin standard biological sample. Background technique [0002] Tetrodotoxin (TTX) is a highly toxic non-protein neurotoxin that exists in pufferfish (Tetraodontidae) and other marine organisms. As an analgesic and anesthetic, it is widely used in medicine, and TTX standards are also required in the field of aquatic animal food testing. Due to the difficulties in purification and preparation technology and process, high-purity TTX standard products are in short supply. The preparation of TTX mainly relies on the method of extraction from biological materials, and undergoes multi-step purification and concentration steps to obtain high-purity products. [0003] The traditional TTX standard sample preparation process mostly uses the viscera of puffer fish as the extraction raw material, extracts it with an acidic solvent, removes impurities through various chromatography methods, and combines mul...

AI Technical Summary

Problems solved by technology

This method can avoid the problem of wild puffer fish being hunted and killed in large quantities due to the acquisition of raw materials, and at the same time avoids the destruction of puffer fish resources and ecological balance, and is not limited by seasons and regions. However, the content of TTX produced by bacteria is low, which is currently practical. Difficult to apply on a large scale in production

[0004] The chemical structure of TTX is relatively complex (see attached figure 1 ), artificial synthesis is relatively difficult, and there are not many studies on artificial synthesis of TTX technology

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