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Detection reagent for detecting animal haematocyte and detection method

A technology for detecting reagents and animals, which can be used in measurement devices, color/spectral property measurement, and material analysis by observing the effect of chemical indicators. It can solve the problems of inability to classify white blood cells and inaccurate analysis.

Inactive Publication Date: 2018-11-23
浙江亚培生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, human blood cell analyzers and corresponding reagents cannot be directly applied to animal blood cell analysis. In this way, the analysis is very inaccurate, especially the classification of white blood cells cannot be achieved.

Method used

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  • Detection reagent for detecting animal haematocyte and detection method
  • Detection reagent for detecting animal haematocyte and detection method
  • Detection reagent for detecting animal haematocyte and detection method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] Diluent

[0032]

[0033] pH: 7.8, conductivity: 13.4ms / cm, osmotic pressure: 250mOsm / kg;

[0034] hemolytic agent

[0035]

[0036]

[0037] pH: 5.2, conductivity: 14.0ms / cm, osmotic pressure: 280mOsm / kg;

[0038] Dilution ratio, diluent: hemolytic agent = 2ml: 1ml, whole blood: 13μl.

example 2

[0040] Diluent

[0041]

[0042] pH: 7.8, conductivity: 13.4ms / cm, osmotic pressure: 250mOsm / kg;

[0043] hemolytic agent

[0044]

[0045] pH: 5.2, conductivity: 14.0ms / cm, osmotic pressure: 280mOsm / kg;

[0046] Dilution ratio, diluent: hemolytic agent = 2ml: 1ml, whole blood: 13μl.

[0047] Due to the influence of seasons, the physiological difference between animal blood in summer and winter is relatively large, and blood cells in winter are more resistant to hemolytic agents than in summer. Therefore, it is better to use pre-dilution to measure white blood cell classification in summer.

[0048] Taking the dog as an example, the comparison of blood cell microscope counts and blood cell analyzer counts is as follows:

[0049]

[0050] Note: WBC: total white blood cells, L: lymphocytes, N: neutrophils, M: monocytes, E: eosinophils, B: basophils.

[0051] The comparative data of hemocytometer and microscope detection are as follows:

[0052] Total white blood cell...

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PUM

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Abstract

The invention discloses a reagent for realizing white cell three-way classification and detecting red cells, white cells and blood platelets in animal blood by an electric-resistivity method haematocyte instrument. The reagent is prepared from diluent and a hemolytic agent, wherein the diluent is used for detecting the red cells and the blood platelets; then, the diluent is matched with the hemolytic agent for detecting the white cells; five kinds of white cells are subjected to three-way classification, and are sequentially divided into lymphocyte group, neutrophilic granulocyte, mononuclearleucocyte groups and acidophilic cell groups according to the volume size. Compared with an ordinary optical microscopic method, the method has the advantage that the result is accurate and reliable.

Description

technical field [0001] The invention belongs to the field of animal disease prevention, treatment and experimental research, in particular to methods and reagents for animal blood count and white blood cell classification. Background technique [0002] Blood cells have a certain resistance in the electrolyte solution. When the two ends of the micropore plate are energized, the electrolyte solution is pumped to flow directionally. When the cells pass through the micropore, the current generates a pulse signal due to the resistance. The size of the pulse is related to the size of the cell. Proportional. The method of the automatic three-differentiation blood cell analyzer is the electrical impedance method, which is divided into two counting pools. A certain diluent is added to the first counting pool to dilute the trace amount of whole blood for the first time, and then the trace amount is absorbed into the second counting pool. A counting pool is used for the second dilutio...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/3103G01N21/78
Inventor 李丰盛陈壮洪骆剑伟
Owner 浙江亚培生物技术有限公司
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