Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mesenchymal stem cell preparation for repairing articular cartilage damage/defect, preparation method and application thereof

A technology of mesenchymal stem cells and articular cartilage, applied in the field of mesenchymal stem cell preparations and their preparation, can solve the problems of weakened cell differentiation function state, cell concentration and quality affecting repair effect, etc., and achieve the effect of promoting repair

Active Publication Date: 2021-11-30
马琳 +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, injecting directly through the joint cavity is a simple and easy operation method, but the directly injected MSCs may cause the number of transplanted cells to decrease continuously and the state of cell differentiation function to weaken due to reasons such as not adapting to the microenvironment of the joint cavity, while the cell concentration and The quality directly affects the repair effect, so multiple injections of transplanted cells are required to improve the curative effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mesenchymal stem cell preparation for repairing articular cartilage damage/defect, preparation method and application thereof
  • Mesenchymal stem cell preparation for repairing articular cartilage damage/defect, preparation method and application thereof
  • Mesenchymal stem cell preparation for repairing articular cartilage damage/defect, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preliminary Example 1 Isolation and Culture of Synoviocytes Used in Co-Culture

[0031] 1) Screen patients without immune diseases such as rheumatism, rheumatoid and osteoarthritis, and collect healthy synovial tissue from the knee joint of patients with meniscus or cruciate ligament injury or synovial donors through arthroscopic surgery, and transfer the tissue to Stored in PBS buffer containing 1% double antibody.

[0032] 2) Under aseptic conditions, the synovium tissue was placed in a petri dish and shredded, washed 3 times with PBS buffer solution, and collected into a centrifuge tube.

[0033] 3) Add 4% type I collagenase 5 times the volume of the tissue, place it in a constant temperature shaker, and digest at 150 rpm at 37°C for 2-3 hours.

[0034] 4) After the tissue block was completely digested, the cell suspension was filtered through a 70 μm cell strainer, and the collected filtrate was centrifuged at 1500 rpm for 5 min.

[0035] 5) Discard the supernatan...

Embodiment 2

[0057] The preparation of embodiment 2 preparations

[0058] The 11 groups of co-cultured umbilical cord mesenchymal stem cells cryopreserved in Example 1 were treated as follows: resuscitated in a 37°C water bath, washed once with normal saline, centrifuged at 2000rpm for 5min, and the supernatant was discarded.

[0059] 1) Resuspend the cells with normal saline as an adjuvant, and adjust the cell concentration to 1×10 6 / mL, 1mL each, inhaled into a syringe for use.

[0060] 2) Resuspend the cells with physiological saline as an adjuvant, and adjust the cell concentration to 10×10 6 / mL, 1mL each, inhaled into a syringe for use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a mesenchymal stem cell preparation for repairing articular cartilage damage / defect, a preparation method and application thereof. In the present invention, mesenchymal stem cells and synoviocytes are indirectly co-cultured, and the co-cultured mesenchymal stem cells are harvested and frozen; The obtained umbilical cord mesenchymal stem cells are more likely to differentiate towards cartilage under the same induction conditions. The present invention confirms that the umbilical cord mesenchymal stem cells co-cultured with synoviocytes have an obvious effect in promoting the repair of articular cartilage damage / defect through the constructed animal knee articular cartilage damage / defect model. The present invention further provides a preparation prepared by using the co-cultured mesenchymal stem cells, which can be applied to clinical repair of articular cartilage damage / defect, and the preparation can effectively promote the repair of articular cartilage damage / defect through joint cavity injection, and treat The effect is obviously better than that of mesenchymal stem cell preparation alone.

Description

technical field [0001] The present invention relates to a preparation for repairing articular cartilage damage / defect, in particular to a mesenchymal stem cell preparation for repairing articular cartilage damage / defect and a preparation method thereof, and the present invention further relates to the medicinal use of the mesenchymal stem cell preparation, namely repairing articular cartilage The damage / defect belongs to the field of mesenchymal stem cell preparation and its preparation and application. Background technique [0002] Articular cartilage is a layer of hyaline cartilage covering the joint surface, composed of chondrocytes and matrix. The surface of articular cartilage is smooth, which can reduce friction, protect joints from wear and tear while bearing sports mechanical loads, and play an important role in joint activities. The main causes of articular cartilage damage / defect include long-term heavy and high-load exercise and middle-aged and elderly osteoarthr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/077A61K35/28A61P19/02
CPCA61K35/28A61P19/02C12N5/0668C12N2502/13
Inventor 张仲文刘爱兵刘莹
Owner 马琳
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com