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Method for innovatively preparing new variety of TYLCV (tomato yellow leafcurl virus)-resisting solanum lycopersicum by gene editing and application thereof

A tomato yellowing curved leaf, gene editing technology, applied in chemical instruments and methods, biochemical equipment and methods, applications, etc., can solve the problems of insufficient antigens of anti-TYCLV materials, loss of resistance of disease-resistant materials, long breeding time, etc. , to achieve the effects of reducing the spread of diseases, good horticultural traits, and reducing the amount of application

Inactive Publication Date: 2018-11-16
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

[0008] The present invention aims at the problem of insufficient antigens in existing anti-TYCLV materials in tomato, the problem of loss of resistance in the production of disease-resistant materials, and the problems of long time and high cost of conventional breeding, and gene editing of the Ty-5 gene by means of gene editing , quickly endow the susceptible material with resistance to TYLCV while retaining the horticultural traits of the original susceptible material

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  • Method for innovatively preparing new variety of TYLCV (tomato yellow leafcurl virus)-resisting solanum lycopersicum by gene editing and application thereof
  • Method for innovatively preparing new variety of TYLCV (tomato yellow leafcurl virus)-resisting solanum lycopersicum by gene editing and application thereof
  • Method for innovatively preparing new variety of TYLCV (tomato yellow leafcurl virus)-resisting solanum lycopersicum by gene editing and application thereof

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Embodiment Construction

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0033] The method for creating new tomato germplasm resistant to tomato yellow leaf curl disease by means of gene editing, comprising the following steps:

[0034] (1) Material

[0035] Tomato material: material moneymaker susceptible to tomato yellow leaf curl disease;

[0036] CRISPR / Cas9 kit: CRISPR / Cas9 kit VK005-14 from Beijing Weishang Lide Biotechnology Co., Ltd.;

[0037] T carrier: Beijing Quanshijin Biotechnology Co., Ltd. (CT301-01);

[0038] DNA...

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Abstract

The invention relates to the technical field of crop seed breeding using a gene editing technology, in particular to a method for innovatively preparing a new variety of TYLCV (tomato yellow leafcurlvirus)-resisting solanum lycopersicum by gene editing and application thereof. The method has the advantages that by editing a gene Ty-5, the recovery mechanism of the plant self on DBS (deep brain stimulation) is utilized, the deletion of DNA (deoxyribonucleic acid) sequence is introduced, the function of the gene Ty-5 is lost, and the disease-resistant property on TYLCV is obtained; compared with the conventional seed breeding method, the resistance on the TYLCV by the disease sensing material can be quickly obtained; compared with the conventional seed breeding method, the garden characterof the receptor material can be well reserved.

Description

technical field [0001] The invention relates to the technical field of crop breeding by gene editing technology, in particular to a method for creating new tomato germplasm resistant to tomato yellow leaf curl virus disease through gene editing and its application. Background technique [0002] Tomato (Solanum lycopersicum) is an important vegetable crop in the world, which plays a huge role in promoting agricultural economic development and increasing farmers' income. The planting process is susceptible to various diseases, resulting in a decrease in yield, so it is very important to breed disease-resistant varieties. [0003] Tomato yellow leaf curl disease (Tomato yellow leaf curl disease TYLCD) is an important disease of tomato, which is caused by tomato yellow leaf curl virus (Tomato yellow leaf curl virus) virus TYLCV) and is mainly transmitted by whitefly (Bemisiatabaci). The disease was first reported in Israel. With the deepening of international trade, TYLCV grad...

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Application Information

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IPC IPC(8): C12N15/82A01H5/00A01H6/82
CPCC07K14/415C12N15/8213C12N15/8283C12N2810/10
Inventor 王银磊赵统敏余文贵赵丽萍周蓉宋刘霞
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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