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Core-shell structure microbial preparation for soil restoration and preparation method and application thereof

A technology of microbial preparation and core-shell structure, applied in the field of soil remediation, can solve problems such as poor remediation effect, difficult remediation, environmental pollution, etc., and achieve the effect of being conducive to popularization and utilization and outstanding effect.

Active Publication Date: 2018-10-09
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, soil heavy metal pollution has the characteristics of concealment, accumulation, hysteresis and long-term nature. It is difficult to repair and the repair effect is not good. Therefore, heavy metal pollution is the focus of environmental science and technology research.
[0004] At present, the selection of microorganisms for soil remediation has become a hot spot in environmental governance research. However, since microorganisms are not easy to survive in harsh environments, this research has become the main obstacle in addition to environmental pollution. In order to enable microorganisms to have better adaptability , it is urgent to study the effective protection of microorganisms and the effective treatment of environmental pollution

Method used

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  • Core-shell structure microbial preparation for soil restoration and preparation method and application thereof

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Effect test

Embodiment 1

[0029] A microbial preparation with a core-shell structure for soil remediation. The microbial preparation with a core-shell structure is composed of particles with a core-shell structure. The particles with the core-shell structure include a composite preparation and an outer shell attached to the surface of the composite preparation; It includes Rhodopseudomonas palustris DSM5859 inoculum, Lactobacillus plantarum NJ2205 inoculum, and Bacillus subtilis BNCC188062 inoculum; the outer shell layer includes the shell layer of the nano hydrogel.

[0030] Wherein, the weight ratio of the compound formulation and the outer shell layer is 0.5:1.

[0031] Specifically, the preparation method of the core-shell microbial preparation for soil remediation is as follows:

[0032] 1) Inoculate Rhodopseudomonas palustris DSM5859 into the seed culture medium and cultivate it to the logarithmic phase, and then inoculate the bacteria cultured to the logarithmic phase into the fermentation medium at an...

Embodiment 2

[0038] A microbial preparation with a core-shell structure for soil remediation. The microbial preparation with a core-shell structure is composed of particles with a core-shell structure. The particles with the core-shell structure include a composite preparation and an outer shell attached to the surface of the composite preparation; It includes Rhodopseudomonas palustris DSM5859 inoculum, Lactobacillus plantarum NJ2205 inoculum, and Bacillus subtilis BNCC188062 inoculum; the outer shell layer includes the shell layer of the nano hydrogel.

[0039] Wherein, the weight ratio of the compound formulation and the outer shell layer is 0.5:2.

[0040] Specifically, the preparation method of the core-shell microbial preparation for soil remediation is as follows:

[0041] 1) Inoculate Rhodopseudomonas palustris DSM5859 into the seed culture medium and cultivate it to the logarithmic phase, and then inoculate the bacteria cultured to the logarithmic phase into the fermentation medium at a ...

Embodiment 3

[0047] A microbial preparation with a core-shell structure for soil remediation. The microbial preparation with a core-shell structure is composed of particles with a core-shell structure. The particles with the core-shell structure include a composite preparation and an outer shell attached to the surface of the composite preparation; It includes Rhodopseudomonas palustris DSM5859 inoculum, Lactobacillus plantarum NJ2205 inoculum, and Bacillus subtilis BNCC188062 inoculum; the outer shell layer includes the shell layer of the nano hydrogel.

[0048] Wherein, the weight ratio of the compound formulation and the outer shell layer is 0.5:1.5.

[0049] Specifically, the preparation method of the core-shell microbial preparation for soil remediation is as follows:

[0050] 1) Inoculate Rhodopseudomonas palustris DSM5859 into the seed culture medium and cultivate it to the logarithmic phase, and then inoculate the bacteria cultured to the logarithmic phase into the fermentation medium at ...

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Abstract

The invention discloses a core-shell structure microbial preparation for soil restoration. The core-shell structure microbial preparation is composed of grains of a core-shell structure, the grains ofthe core-shell structure comprise a composite preparation and an outside shell layer adhered on the surface of the composite preparation, the composite preparation comprises rhodopseudomonas palustris DSM5859 bactericide, lactobacillus plantarum NJ2205 bactericide and bacillus subtilis BNCC188062 bactericide, and the outside shell layer comprises a shell layer of nano hydrogel. The invention further discloses a preparation method and application of the core-shell structure microbial preparation. In soil polluted by heavy metal, pH of the soil changes accordingly; in order to enable the microbial preparation to adapt to and treat heavy metal pollution more effectively, a layer of nano hydrogel capable of tolerating pH is coated on the surface of a microbial agent, and ability of removing heavy metal is improved greatly through synergism of the microbial agent and the nano hydrogel.

Description

[0001] Technical solutions [0002] The invention belongs to the technical field of soil remediation, and specifically relates to a core-shell structure microbial preparation used for soil remediation, and a preparation method and application thereof. Background technique [0003] With the acceleration of urbanization and industrialization, the degree of heavy metal pollution in the soil near cities and industrial areas is also increasing, and the pollution area is gradually expanding. According to reports, my country's heavy metal contaminated soil has exceeded 20 million hectares. 2 . Moreover, soil heavy metal pollution has the characteristics of concealment, accumulation, lagging and long-term, and it is difficult to repair and the repair effect is not good. Therefore, heavy metal pollution is the focus of environmental science and technology research. [0004] At present, the selection of microorganisms for soil remediation has become a hot spot in environmental governance resea...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/08B09C1/10C12R1/38C12R1/25C12R1/125
CPCC12N1/20B09C1/08B09C1/105B09C2101/00
Inventor 谢悦波陈求稳何庆成金波姚竣耀
Owner 江苏世邦生物工程科技有限公司
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