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Large-scale production and culture method for mammalian cells

Technology of a mammal, culture method

Inactive Publication Date: 2018-10-09
GUANGDONG ANNPO BIOTECHNOLOGY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For mammalian cell culture processes with high foam volume, increasing the amount of defoamer used can achieve high quality and high yield, but it will increase the difficulty and risk of downstream drug development

Method used

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  • Large-scale production and culture method for mammalian cells
  • Large-scale production and culture method for mammalian cells
  • Large-scale production and culture method for mammalian cells

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Embodiment Construction

[0023] The large-scale production and cultivation method of mammalian cells described in the present invention is composed of cell resuscitation and shake flask expansion step, tank expansion step and tank production step, wherein the tank production step is carried out in a disposable bioreactor . The structure of the disposable bioreactor bag of the disposable bioreactor is as follows: figure 1 and figure 2 shown. The disposable bioreactor bag adopts the Xcellerex-XDR type disposable bioreactor bag structure, and the top of the disposable bioreactor bag body 1 has two ventilation ports 11, one of which is connected to the defoamer adding component 2, and the other The ventilation interface is connected to the foam drainage assembly 3 .

[0024] The defoamer adding assembly 2 is mainly composed of a tee 21 , an exhaust filter 22 , a container 23 with a built-in defoamer and a peristaltic pump 24 . The first interface of the tee 21 is connected with a ventilation interfac...

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Abstract

The invention discloses a large-scale production and culture method for mammalian cells. The method consists of a cell recovery and shake flask propagation step, a tank propagation step and a tank production step in sequence, wherein the tank production process is carried out in a disposable bioreactor; at the peak of foam production, the exhaust flow is controlled at 80 to 100 percent of the exhaust flow; 0.01 to 0.1 percent by mass of defoamer is added; counted by the total volume 140L of cell culture liquid after cell inoculation in the tank production step, the adding speed of the defoameris 2.5 to 10ml per hour. By adopting the disposable bioreactor for large-scale production of mammalian cells with high foam content, high quality and high yield can be realized, the total adding amount of the defoamer can be controlled effectively, and smooth proceeding of production and culture is ensured.

Description

technical field [0001] The invention belongs to the technical field of cell production and cultivation, and in particular relates to a large-scale production and cultivation method of mammalian cells. Background technique [0002] At present, the equipment used for large-scale cell culture in industry is mainly stainless steel cell tanks and disposable bioreactors. Stainless steel cell tanks can replace / increase / decrease different accessories according to cell growth and production characteristics to achieve high-quality and high-yield cells. However, stainless steel cell tanks have been replaced by disposable bioreactors in recent years due to the adverse effects of high use and maintenance costs, complicated pipeline construction, and increased cleaning and verification procedures. The disposable bioreactor is mainly composed of a fixed support structure, a controller and a disposable bioreactor bag. Disposable bioreactor bags are disposable sterile products and do not n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/21
CPCC12M23/14C12M41/02
Inventor 贺炜华邓新宇
Owner GUANGDONG ANNPO BIOTECHNOLOGY INC
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