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Application of rice osgy1376t mutant material in breeding direct seeding varieties with high seedling emergence rate

A technology of rice and varieties, applied in the direction of application, hydrolase, determination/inspection of microorganisms, etc.

Active Publication Date: 2021-08-10
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have shown that there is no correlation between mesocotyl length and adult plant height

Method used

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  • Application of rice osgy1376t mutant material in breeding direct seeding varieties with high seedling emergence rate
  • Application of rice osgy1376t mutant material in breeding direct seeding varieties with high seedling emergence rate
  • Application of rice osgy1376t mutant material in breeding direct seeding varieties with high seedling emergence rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049]Example 1. Obtaining of gy1 mutant and its phenotype analysis and identification of mutant gene sequence

[0050] 1. Discovery and phenotypic analysis of gy1 mutants

[0051] 1. Discovery of gy1 mutants

[0052] In the process of screening rice ethylene response-deficient mutants, the present invention found a mutant etiolated seedling derived from Nipponbare, whose coleoptile was longer than its control Nipponbare (Oryza sativa L.cv.Nipponbare, Nip), and had hydroponic conditions The mesocotyl structure was difficult to be observed in the yellow seedlings of Nipponbare, and the mutant was named gy1.

[0053] 2. Detection of coleoptile and mesocotyl length under hydroponic conditions

[0054] Under hydroponic conditions, both the coleoptile and mesocotyl of gy1 were significantly longer than those of the control Nipponbare (Nip). The coleoptile length of gy1 was about 21 mm, and that of the control Nipponbare was about 13 mm. It was difficult for Nipponbare to detect ...

Embodiment 2

[0072] Example 2, OsGY1 gene SNP site G376T and OsGY1 376T Gene acquisition

[0073] Through the screening of 52 varieties, it was found that the rice variety Casalas also has the characteristic of long mesocotyl. By detecting the coleoptile and mesocotyl lengths of rice varieties Kasaras and gy1 mutants, it was found that the coleoptile lengths of the gy1 mutant and Kasaras were about 1.6 and 6.0 mm, and the mesocotyl lengths were 19.5 mm, respectively. and 24.0 mm ( Figure 5 ). Both the coleoptile and mesocotyl of the rice variety Kasaras (Kas) were longer than those of the gy1 mutant.

[0074] Further sequencing of the Casalas OsGY1 gene showed that the 376th nucleotide of the Casalas OsGY1 gene was mutated from G to T, resulting in a mutation of the 126th amino acid of the OsGY1 protein encoded by it from glycine to cysteine ​​(R126C )( Figure 6 ), the site (the 376th nucleotide of the OsGY1 gene) was named G376T. The mutated OsGY1 gene sequence is shown in sequenc...

Embodiment 3

[0075] Example 3, Correlation analysis of OsGY1 gene SNP site G376T and rice mesocotyl length

[0076] 1. OsGY1 genotype detection

[0077] After the 376th nucleotide of OsGY1 gene was mutated from G to T in Kasaras, the length of the coleoptile and mesocotyl was elongated. Correlation. Meta-analysis was performed on the OsGY1 gene SNP site G376T in the public resequencing data of 3000 rice resources with diverse representatives, and the genotype (TT genotype, GG genotype or GT genotype) of each rice resource was obtained.

[0078] Compared with the OsGY1 gene sequence of wild-type rice Nipponbare, it was found that 141 rice resources (including Kasalath, Kas) from different countries and regions belonged to different rice subgroups among the 3000 rice resources. It is TT genotype, that is, the 376th nucleotide of OsGY1 gene is mutated from G to T.

[0079] 2. Correlation analysis between genotype of OsGY1 gene SNP site G376T and mesocotyl phenotype

[0080] On the basis o...

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Abstract

The invention discloses the application of rice OsGY1376T mutation material in cultivating varieties with high seedling emergence rate through direct seeding. Through the screening of 52 rice varieties, the present invention finds that rice varieties such as Casalas have the characteristics of long mesocotyls, and obtains the SNP sites G376T and OsGY1 of the OsGY1 gene related to the length of the mesocotyls 376T Gene, the homozygous mutation of the SNP site from G to T leads to the elongation of the mesocotyl and the coleoptile, and does not affect other agronomic traits of rice. The invention not only has great significance for cultivating direct-seeding rice varieties and solving the time-consuming and labor-consuming problems of rice transplanting, but also provides a new idea for improving the emergence rate of rice seedlings in direct-seeding rice breeding.

Description

technical field [0001] The invention relates to the application of rice OsGY1376T mutation material in cultivating varieties with high seedling emergence rate through direct seeding, and belongs to the field of conventional rice breeding. Background technique [0002] Rice is the most important food crop in my country. In the actual production process, rice transplanting consumes a lot of manpower and time, so direct seeding cultivation of rice has gradually attracted attention. [0003] Rice seedlings are mainly composed of roots, mesocotyls, coleoptiles and true leaves. Among them, the mesocotyl and the coleoptile are separated by the coleoptile node between the two, on which the adventitious root grows. The lengths of rice mesocotyls and coleoptiles vary under different seedbed humidity conditions. Under the most suitable humidity conditions, most indica rice varieties have longer mesocotyls and coleoptiles, but some japonica rice varieties do not have obvious changes i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N9/16C12N15/55A01H1/02A01H1/04
CPCA01H1/02A01H1/04C12N9/16C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 张劲松陈受宜熊青马彪张万科何锶洁林晴
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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