Method and system for analyzing interaction relationship between populus tomentosa LncRNA Pto-CRTG and target gene Pto-CAD5 thereof by utilizing epistasis
A technology of lncrnapto-crtg and pto-cad5, applied in the field of genetics research
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[0080] Raw material acquisition: the individuals with the highest growth and the lowest growth of Populus tomentosa came from a hybrid population of Populus tomentosa (Populus tomentosa “TB14” × Populus tomentosa “ Pt-3"); the natural population of Populus tomentosa used for association mapping analysis comes from the national P. tomentosa germplasm resource bank, which represents the natural distribution of P. tomentosa across the country;
[0081] Reagents used in the polymerase chain reaction (PCR) system were purchased from Promega;
[0082] Combine Tophat and Cufflink package system for lncRNA prediction and analysis;
[0083] Epistasis association analysis was performed using EPISNP software.
[0084] The specific operation steps are as follows:
[0085] Step S1, in the Populus tomentosa hybrid population composed of 736 individuals of Populus tomentosa, according to the comprehensive evaluation results of tree height, diameter at breast height and volume, respectively...
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