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A Strong Stability Model for Nonlinear Multistage Enzyme Catalytic Systems

A technology with strong stability and catalytic system, applied in the field of bioengineering, can solve problems that do not consider different effects

Active Publication Date: 2021-04-13
HUZHOU TEACHERS COLLEGE
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Problems solved by technology

In recent years, some people have established a gene regulatory network for the dha regulator in the glycerol metabolism process, given a quantitative analysis model for global regulation of gene expression, and achieved results in the identification of path parameters for the hybrid system of enzyme catalysis and gene regulation in batch fermentation. They did not consider the different effects of cells at different stages in the control process, so how to have different characteristics of reactants at different stages, and how to increase the concentration of batch fermentation products at different stages have become problems that need to be solved

Method used

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  • A Strong Stability Model for Nonlinear Multistage Enzyme Catalytic Systems
  • A Strong Stability Model for Nonlinear Multistage Enzyme Catalytic Systems
  • A Strong Stability Model for Nonlinear Multistage Enzyme Catalytic Systems

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Embodiment Construction

[0062]Glycerol intermittent fermentation produces 1,3-Pd to three different stagesThat is, the development stageOr the first stage), growth phaseOr second phase) and stable stagesOr the third phase). makeFor time parameters, satisfy:

[0063]Order iNN = 1, 2, ..., n is the sequence number set of experiments in discrete culture, where n represents all of the tests.Represents L∈iiiNThe initial state of the first phase of the second experiment.Represents L∈iiiNThe end state of the first phase I-1 stage is also the initial state of the first phase.

[0064]The following nonlinear multi-stage power system is proposed:

[0065]

[0066]among themIt is system parameter variable in the i-th phase of the system.L∈iiiNState variables in the i-phase of the experiment. among themRespectivelyDistance T∈D, respectivelyi∈ii3Time fermentation tanks, extracellular glycerol, extracellular 1,3-Pd, acetic acid, intracellular glycerol, intracellular hydroxypropionate (branch with 3-HPa) and intracellular 1,3-Pd con...

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Abstract

The present invention relates to a strong stability model of a nonlinear multi-stage enzyme catalysis system. The model uses an optimization method to reveal that reactants have different characteristics in different batch fermentation stages. The concentration of extracellular 1,3-propanediol is Performance indicators, taking the hybrid nonlinear dynamical system, the approximate stability of the batch fermentation system, the relative error of the concentration of intracellular and extracellular substances, and the biological robustness of the concentration of intracellular substances as the main constraints, a strong stability model was constructed to study a class of A nonlinear dynamical system that cannot find an analytical solution and has no equilibrium point. The present invention establishes a nonlinear multi-stage power system and transforms it into a linear variational equation, constructs a method for linear processing of nonlinear problems, better solves the nonlinear multi-stage power system, and proposes a nonlinear multi-stage intermittent The conditions of the strong stability model of the enzyme fermentation catalytic power system are calculated by the control model to maximize the output of the system.

Description

Technical field[0001]The present invention belongs to the field of bio-engineering technology, and is specifically involved in a strong stability model for nonlinear multi-stage enzyme catalytic systems.Background technique[0002]Glycerol fermentation is mainly divided into three types: intermittent fermentation, continuous fermentation and batch flow fermentation. The research results are mainly concentrated in continuous and intermittent fermentation process. If people use the corrected excessive dynamics model, the continuous and intermittent fermentation process is described, predicting the polymorphism in the fermentation process; proposing glycerol creatures including three substances The reduction pathway enzyme catalytic model of the 1,3-PD process, in addition to describing the variation of the extracellular substance, the catalytic effect on the two major enzymes on the intracellular reduction path is considered. In recent years, some people have established a gene regulato...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G05B13/04
CPCG05B13/042
Inventor 王金鹤庞丽萍王帅吕佳佳
Owner HUZHOU TEACHERS COLLEGE
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