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Three-branch-like nucleic acid nano silver fluorescent cluster as well as preparation method and application

A nucleic acid nano and fluorophore technology, applied in the field of biofunctional fluorescent nanomaterials, can solve problems such as the inability to regulate the performance of nanosilver clusters, achieve strong fluorescence effects, improve sensitivity, and expand the application range

Inactive Publication Date: 2018-08-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nucleic acid has good biocompatibility, but current research only uses single-stranded nucleic acid to synthesize silver nanomaterials, and cannot regulate the properties of silver nanoclusters, such as the regulation of stability and fluorescence.

Method used

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  • Three-branch-like nucleic acid nano silver fluorescent cluster as well as preparation method and application
  • Three-branch-like nucleic acid nano silver fluorescent cluster as well as preparation method and application
  • Three-branch-like nucleic acid nano silver fluorescent cluster as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing a three-dendritic nucleic acid nano-silver fluorophore cluster, comprising the steps of:

[0030] (1) According to the principle of complementary base pairing, design and synthesize three oligonucleotides, which are used to form three branched DNAs with the same sticky ends in each branch;

[0031] The sequences of the three oligonucleotides are:

[0032] SEQ ID NO.1:

[0033] 5'- -3'

[0034] SEQ ID NO.3:

[0035] 5'- -3'

[0036] SEQ ID NO.5:

[0037] 5'- -3'

[0038] The sequence consisting of 22 nucleotides of the 5' of the above three sequences is a cohesive end;

[0039] The sequence (marked with dotted line) formed by the 23rd-36 nucleotides of the SEQ ID NO.1 sequence is reverse complementary to the sequence (marked with the dotted line) formed by the 37th-50 nucleotides of the SEQ ID NO.5 sequence :

[0040] The sequence (marked with double lines) that the 37th-50 nucleotides of SEQ ID NO.1 sequence consists of and the seque...

Embodiment 2

[0047] A method for preparing a three-dendritic nucleic acid nano-silver fluorophore cluster, comprising the steps of:

[0048] (1) According to the principle of complementary base pairing, three oligonucleotides are designed and synthesized: for forming three branched DNAs with the same cohesive ends in each branch;

[0049] The sequences of the three oligonucleotides are:

[0050] SEQ ID NO.2:5'-ACCCTTACCCTTACCCTTACCC -3'

[0051] SEQ ID NO.4: 5'-ACCCTTACCCTTACCCTTACCC -3'

[0052] SEQ ID NO.6:5'-ACCCTTACCCTTACCCTTACCC -3'

[0053] The sequence consisting of 22 nucleotides of the 5' of the above three sequences is a cohesive end;

[0054] The sequence (marked with dotted line) consisting of the 23rd-36 nucleotides of the SEQ ID NO.2 sequence is reverse complementary to the sequence (marked with the dotted line) composed of the 37th-50 nucleotides of the SEQ ID NO.6 sequence :

[0055] The sequence (marked with a double line) of the 37th-50 nucleotides of the...

Embodiment 3

[0062] A method for preparing a three-dendritic nucleic acid nano-silver fluorophore cluster, comprising the steps of:

[0063] (1) According to the principle of complementary base pairing, three oligonucleotides are designed and synthesized: for forming three branched DNAs with the same cohesive ends in each branch;

[0064] The sequence of three oligonucleotides is the same as the sequence of three oligonucleotides of embodiment 1;

[0065] (2) Put the synthesized three oligonucleotides into water respectively, shake at room temperature to obtain 100 μM aqueous solution of the first oligonucleotide (SEQ ID NO.1), the second oligonucleotide acid (SEQ ID NO.3) aqueous solution and the third oligonucleotide (SEQ ID NO.5) aqueous solution;

[0066] (3) Take the first oligonucleotide aqueous solution, the second oligonucleotide aqueous solution and the third oligonucleotide aqueous solution with a volume of 30 μL and a concentration of 100 μM, and 10 μL of an aqueous sodium chlo...

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Abstract

The invention discloses a three-branch-like nucleic acid nano silver fluorescent cluster as well as a preparation method and an application. The preparation method comprises the steps as follows: (1)three oligonucleotides are designed and synthesized according to a base complementary pairing principle to be used for forming three-branch-like DNA with the same sticky ends on all branches; (2) thethree oligonucleotides are prepared into corresponding aqueous solutions; (3) a three-branch-like DNA solution with the same sticky ends on all branches is prepared; (4) the three-branch-like DNA solution with the same sticky ends on all branches, a silver nitrate aqueous solution, a phosphate buffered solution and a sodium borohydride aqueous solution are stirred, and the three-branch-like nucleic acid nano silver fluorescent cluster is obtained. The three-branch-like nucleic acid nano silver fluorescent cluster is stronger in fluorescent effect and stable, the sensitivity of a fluorescent material in analysis and detection is improved, and the application range of the nucleic acid nano silver fluorescent material is expanded. The nucleic acid nano silver fluorescent cluster can detect Dtype amino acid.

Description

technical field [0001] The invention belongs to the technical field of biological functional fluorescent nanomaterials, and in particular relates to a three-dendritic nucleic acid nano-silver fluorescent cluster and a preparation method thereof. Background technique [0002] With the continuous development of nanoscience and biotechnology, the application of nanofluorescent materials in environmental detection, biometric detection, bioimaging labeling, biocatalysis and other fields has become more and more extensive, which has attracted great attention from governments and academic circles around the world. [0003] Traditional D-amino acid analysis and detection methods include optical analysis, electrochemical analysis, nuclear analysis, chromatographic analysis, and electron microscope analysis. The above method has been used for the detection of target objects, with low detection line and good repeatability, but it needs to rely on large-scale analytical instruments and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/58B22F9/24B82Y40/00B82Y30/00G01N21/64
CPCB22F9/24B82Y30/00B82Y40/00C09K11/58G01N21/6486
Inventor 仰大勇杨璐
Owner TIANJIN UNIV
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