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High-efficiency detection method for asymmetric dimethylarginine

A technology of dimethylarginine and detection method, which is applied in the field of high-efficiency detection of asymmetric dimethylarginine, can solve the problem of not obtaining asymmetric dimethylarginine ADMA detection method, low throughput and cost higher question

Active Publication Date: 2021-03-02
HANGZHOU BAICHEN MEDICAL LAB CO LTD +1
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Problems solved by technology

[0004] At present, clinical methods for the determination of asymmetric dimethylarginine ADMA mainly include high-pressure liquid chromatography, enzyme-linked immunoassay, and immunoturbidimetric assay, etc., but high-pressure liquid chromatography has the problem of insufficient sensitivity. Both the combined immunoassay and the immunoturbidimetric assay need to configure specific kits, and there are problems of complicated operation, low throughput and high cost
[0005] All in all, there is no generally accepted clinical detection method for asymmetric dimethylarginine ADMA

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  • High-efficiency detection method for asymmetric dimethylarginine

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[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present invention belong to the protection scope of the present invention.

[0041] Those skilled in the art should understand that in the disclosure of the present invention, the terms "vertical", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present invention and The above terms should not be con...

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Abstract

The invention discloses a high-efficiency detection method for asymmetric dimethylarginine, which is suitable for detecting the content of asymmetric dimethylarginine in a sample, comprising the following steps: obtaining asymmetric dimethylarginine quantitative standard correction Curve; preparation of detection product; separation of asymmetric dimethylarginine by high-throughput liquid chromatography; detection of said asymmetric dimethylarginine by tandem mass spectrometry; and method for obtaining said asymmetric dimethylarginine The high-efficiency detection method for asymmetric dimethylarginine uses high-throughput liquid chromatography tandem mass spectrometry to quickly and efficiently detect the content of asymmetric dimethylarginine in biological samples.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a high-efficiency detection method for asymmetric dimethylarginine, wherein the high-efficiency detection method for asymmetric dimethylarginine is suitable for quickly and efficiently detecting asymmetric dimethylarginine in biological samples. content of methyl arginine. Background technique [0002] Asymmetrical dimethylarginine (ADMA) is a natural amino acid widely present in tissues and cells. It uses S-adenosylmethionine as a methyl donor and is transferred by methylated proteins. The enzyme (Proteinarginine methyltransferase, PRMT) catalyzes arginine residues in proteins or polypeptides, making these arginine residues methylated to form methylated proteins, and then hydrolyzed from methylated proteins under the action of proteolytic enzymes Come. It is excreted unchanged by the kidneys and is mostly metabolized by dimethylarginine dimethylaminohydrolase. ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 吴超超胡志飞高强彭军
Owner HANGZHOU BAICHEN MEDICAL LAB CO LTD
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