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Application of 5-hydroxymethylcytosine and 5-formylcytosine in prognosis evaluation of hepatocellular carcinoma

A technology of hydroxymethylcytosine and formylcytosine, applied in the application field of 5-hydroxymethylcytosine and 5-formylcytosine in the prognosis assessment of hepatocellular carcinoma, which can solve the problem of reducing RNA polymerase II transcription Efficiency and specificity of binding

Active Publication Date: 2018-07-24
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Problems solved by technology

Moreover, 5fC and 5caC may also reduce the transcription efficiency and binding specificity of RNA polymerase II

Method used

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  • Application of 5-hydroxymethylcytosine and 5-formylcytosine in prognosis evaluation of hepatocellular carcinoma
  • Application of 5-hydroxymethylcytosine and 5-formylcytosine in prognosis evaluation of hepatocellular carcinoma
  • Application of 5-hydroxymethylcytosine and 5-formylcytosine in prognosis evaluation of hepatocellular carcinoma

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Embodiment 1

[0043] 1. Materials and methods

[0044] 1. Patient and Tissue Samples

[0045] Paired primary HCC samples and their adjacent normal liver tissues were obtained from 106 patients undergoing surgery at Shanghai Oriental Hepatobiliary Surgery Hospital, China, and patient files and diagnostic information were obtained from the hospital file system and telephone follow-up. The detailed clinical and pathological information of the patients can be seen in Table 1. This study has been approved by the Ethics Committee of the Eastern Hepatobiliary Surgery Hospital, and the informed consent of the patients was obtained before the operation.

[0046] Table 1 Clinicopathological features of the patients

[0047]

[0048]

[0049] Note. a The clinical indicators of a small number of patients are missing.

[0050] 2. Cell Culture

[0051] at 37 ° °C and 5% CO 2 Cells were cultured in DMEM medium containing 10% fetal bovine serum. When the cell confluency is 80-90%, trypsinize a...

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Abstract

The invention relates to the biotechnical field, and concretely relates to an application of 5-hydroxymethylcytosine and 5-formylcytosine in the preparation of a reagent or a kit for prognosis evaluation of hepatocellular carcinoma. It is found that the total genome 5hmC content and the genome 5fC content of HCC tissues are lower than those of cancer-adjacent tissues. Patients with reduced 5hmC and 5fC levels in HCC genome DNA have poor prognosis, and are prone to suffer from recurrence and metastasis. Compared with patients with high 5hmC or 5fC levels, patients with low 5hmC or 5fC levels inHCC tissues have lower disease-free survival and overall survival rates . The application provides a new prognosis evaluation method for the hepatocellular carcinoma.

Description

technical field [0001] The present invention relates to the field of biotechnology, specifically, 5-hydroxymethylcytosine (5-hydroxymethylcysotine, 5hmC) and 5-formylcytosine (5-formylcytosine, 5fC) in hepatocellular carcinoma (hepatocellular carcinoma, HCC) application in prognostic assessment. Background technique [0002] Methylation on the fifth carbon atom of cytosine is an important DNA epigenetic modification (5mC). Abnormal DNA methylation characterized by genome-wide hypomethylation and local hypermethylation is more common in different types of cancer, which is closely related to the initiation and development of tumors. [0003] Studies have shown that the oxidized derivatives of 5mC, namely 5-hydroxymethylcytosine (5-hydroxymethylcysotine, 5hmC), 5-formylcytosine (5-formylcytosine, 5fC) and 5-carboxycytosine (5-carboxylcytosine, 5caC ) is a dynamic process with their own unique regulatory functions, rather than just being an intermediate of DNA demethylation. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/154C12Q2600/118
Inventor 王红阳贺宇飞
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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