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Composition, kit and method for identifying deer species by gexp multiplex PCR

A composition, red deer technology, applied in the biological field, can solve the problem of flux-limited amplification preference and other issues

Active Publication Date: 2022-05-24
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] This technology solves the problems of limited throughput and amplification preference in other technologies

Method used

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  • Composition, kit and method for identifying deer species by gexp multiplex PCR
  • Composition, kit and method for identifying deer species by gexp multiplex PCR
  • Composition, kit and method for identifying deer species by gexp multiplex PCR

Examples

Experimental program
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Effect test

Embodiment 1

[0025] In this example, the specificity, sensitivity and specificity cross-reaction of primers for five deer, red deer, reindeer, elk, fall deer and white-lipped deer were verified.

[0026] The sequences of GeXP multiplex PCR specific chimeric primers were synthesized by American Invitrogen Company. These 5 pairs of specific chimeric primers were based on the original specific primers, and the 5' ends of the upstream and downstream primers were connected with a universal tag. The length of the amplified fragments of the five deer species increased by 37 bp. The specific sequence is shown in Table 1 below.

[0027] Table 1 GeXP-specific chimeric primers and their related information

[0028]

[0029] Note: The underline indicates the universal tag base sequence.

[0030] 1. Gene template extraction:

[0031] DNA extraction from deer hair samples: use the rapid extraction method to extract DNA from deer hair samples containing hair follicles, lysate = 1×Ex Taq Buffer: ...

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Abstract

The invention relates to a GeXP multiplex PCR method for five deer components including red deer, reindeer, elk, fallow deer and white-lipped deer. The invention also relates to oligonucleotide primer probe compositions for use in said methods. The invention also relates to a GeXP multiplex PCR kit comprising said composition. Using the composition of the invention to perform GeXP multiplex PCR detection can simply, quickly, specifically and sensitively detect components of various deer species through one detection.

Description

technical field [0001] The present invention belongs to the field of biotechnology, in particular, the present invention relates to a GeXP multiplex PCR method for qualitatively detecting components of various deer species, an oligonucleotide primer probe composition for the method, and a composition comprising the combination material kit. Background technique [0002] Deer animals and their products have been regarded as the best food for health care since ancient times. In 2000, sika deer and red deer were listed as genuine medicinal materials in the Pharmacopoeia of the People's Republic of China. Since then, the application of deer animals and their products in food, drug and health products has gradually increased, such as antler, antler, antler gum, etc. Venison is also a high-end meat product with high nutritional value. Therefore, deer ingredient adulteration has become an important food inspection item. At present, the methods for species identification at home ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2600/16C12Q2537/143Y02A50/30
Inventor 陈颖吴亚君凌胜男杨艳歌刘鸣畅韩建勋
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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