Large ovarian tissue vitrification freezing carrier and application thereof

Abstract

The invention discloses a large ovarian tissue vitrification freezing carrier. The carrier consists of a carrier inner core and an outer sleeve, wherein the carrier inner core is in threaded connection with the outer sleeve, maintaining high airtightness and preventing cross infection of the ovarian tissue; meanwhile, the user operation is also facilitated; the carrier inner core consists of a handheld part, a cap part, a support part and a bearing part sequentially from top to bottom; the bearing part is clamped and fixed by the support part; when in use, a large ovarian tissue is put on an end part of the bearing part, away from the support part; the end part is a large ovarian tissue loading area; experiments prove that by using the carrier disclosed by the invention, the permeation efficiency of a refrigerant is improved; moreover, the operation is simple, the price is low, batch storage of large ovarian tissues is convenient, and the carrier is suitable for promotion and application.

Description

technical field [0001] The invention belongs to the field of medical equipment, and in particular relates to a bulk ovarian tissue vitrification freezing carrier and application thereof. Background technique [0002] Ovarian tissue cryopreservation is the fastest and most effective way to preserve fertility for adult women undergoing chemotherapy and radiotherapy. For prepubertal women, ovarian tissue cryopreservation is the only way to preserve fertility. The traditional ovarian tissue freezing technology adopts programmed freezing, which has helped many women to have their own children. However, due to the disadvantages of expensive equipment, long freezing time and cumbersome operation, the clinical promotion of ovarian freezing technology is limited. Vitrification technology is the trend of low-temperature freezing of cells / tissues in the future. It has the advantages of simple operation, low cost, and high freezing efficiency. It has basically completely replaced progr...

AI Technical Summary

Problems solved by technology

At present, vitrification carriers used in ovarian cryopreservation mainly include frozen straws, frozen rings, cell mesh, needle-punched carriers, etc. Among them, frozen straws and frozen rings can only load small volumes of ovarian tissue, and needle-punched carriers will Causing irreversible mechanical damage to ovarian tissue, the cell screen occupies too much volume when stored in a liquid nitrogen tank, and it is not easy to store in batches

Therefore, the above-mentioned carriers face many difficulties in the clinical application and promotion of ovarian tissue cryopreservation, and have not been widely accepted.

At present, only the Ova Cryo Device Type M carrier of KITAZATO Company is available on the market, which is not only expensive (about 300 yuan / carrier), but also has a small contact area between the steel needle and the cortex during the ovarian tissue loading process and is easy to fall off. Difficult to popularize at work

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