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A preparation method of macroporous resin used for purification of total polyphenols in Bougainvillea

A technology of macroporous resin and total polyphenols, which is applied in the field of preparation of macroporous resin, can solve the problems that cannot be really applied

Active Publication Date: 2020-11-27
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these experiments have verified the role of macroporous resins in separation and purification, the contents are all below 15%, which cannot be really applied to actual operations

Method used

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  • A preparation method of macroporous resin used for purification of total polyphenols in Bougainvillea
  • A preparation method of macroporous resin used for purification of total polyphenols in Bougainvillea
  • A preparation method of macroporous resin used for purification of total polyphenols in Bougainvillea

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Experimental program
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Embodiment Construction

[0040] 1. Materials and Reagents

[0041] Bougainvillea leaves: collected in Fenghuang Mountain (dried) in Guangdong Province, the thin stems and leaves were taken separately, and crushed for later use. Chlorogenic acid standard substance: self-made in the laboratory, the purity is over 98% verified by HPLC-DAD. Absolute ethanol and petroleum ether were of analytical grade and were purchased from Sinopharm Chemical Reagent Co., Ltd. Folin-Ciocalteu reagent was of analytical grade and was purchased from Shanghai Ruiyong Biotechnology Co., Ltd. Anhydrous sodium carbonate, hydrochloric acid, sodium hydroxide, etc. were purchased from Gaojing Chemical Co., Ltd. for analytical grade. ABTS, DPPH, VC, and VE are analytically pure with a purity of 98%, purchased from Shanghai Macklin Biochemical Technology Co., Ltd. Potassium persulfate was purchased from Tianjin Yongda Chemical Reagent Co., Ltd. for analytical grade. Experimental water: distilled water, self-made in the laborator...

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Abstract

The present invention relates to a method for preparing macroporous resin for purification of total polyphenols in Acanthopanax trifoliatus, and belongs to the technical field of plant-derived pharmaceutical preparations. The method is as follows: immersing macroporous resin in anhydrous ethanol, after the macroporous resin is fully swollen, placing the macroporous resin in a chromatographic column, controlling the resin layer to be free from production of bubbles, loading the anhydrous ethanol onto the chromatographic column for elution, and stopping when eluant, after being added with an equal volume of distilled water, is not turbid; eluting the resin with distilled water until ethanol content is less than 10%; until the distilled water is exhausted, adding lye for soaking for a period of time, eluting the resin with the distilled water until the eluant is neutral, adding an acid solution for soaking for the same period of time, eluting the resin with the distilled water until the eluant is neutral to obtain the treated macroporous resin, and soaking the treated macroporous resin with distilled water in a beaker for standby use. The method is applied to the preparation of the macroporous resin for purification and adsorption, and the macroporous resin has the advantages of high purification efficiency, high polyphenol activity and the like.

Description

technical field [0001] The invention relates to a preparation method of a macroporous resin used for purifying total polyphenols in Bougainvillea, and belongs to the technical field of pharmaceutical preparations containing plants. Background technique [0002] Caffeoylquinic acid is a compound widely present in plants and has pharmacological activities such as anti-oxidation, anti-inflammation, and antibacterial. It is a compound formed by condensation reaction of one molecule of quinic acid and multiple caffeic acids. Zhang et al. determined the content of flavonoids and caffeic acid quinine and other phenolic substances on 24 species of Aralis genus, and confirmed that the leaves of Bougainvillea from different origins are rich in caffeoylquinic acid polyphenols. Kiem et al. reported for the first time that the phenylpropanoid glycosides isolated from the stem bark of Bougainvillea were 1-β-D-glucopyranosyl-2,6-dimethoxy-4-propynyl-ene Alcohol (1) and 1-[β-D-glucopyranos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/26B01J20/30B01J20/34A61K36/254A61P39/06A61K127/00
CPCA61K36/254A61K2236/55B01J20/26B01J20/3475
Inventor 张晓丹侯卓妮梁宗锁汪紫莹陈盛良胡心怡
Owner ZHEJIANG SCI-TECH UNIV
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