Rapid Propagation Method of Ethnic Spice Plant Hemp Root

A spice plant, a fast technology, applied in the biological field, can solve the problems of adult plant damage, no hemp root reproduction, etc., and achieve the effect of strong operability and rapid expansion of the seedling base

Active Publication Date: 2019-05-24
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main propagation method of hemp roots observed in the wild is to use seeds for propagation. However, hemp roots are dioecious plants, and the seedlings seen in the wild are almost all larval plants, and it takes about 5-5 years from larvae to adult plants. For 10 years, the folks mainly eat adult plants, and the damage to adult plants is particularly serious. Almost no flowers and mature seeds of adult plants can be seen in the wild, and it is almost impossible to reproduce with seeds.
This species was officially published as a new species in August 2017, and there is currently no related report on the reproduction of hemp roots

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Rapid propagation of plant hemp root (P.magen B.Q.Cheng ex C.L.long&J.Yang)):

[0019] Including: establishment of sterile clones, optimal selection of medium, screening to promote the formation of cluster buds and rooting culture, among which: the medium for inducing axillary buds from stem segments is ①1 / 4MS+BA0.02mg / L+NAA0.05mg / L ; The medium for inducing the proliferation of young stem cluster buds is ②1 / 4MS+BA0.2mg / L+IAA0.1mg / L and ③1 / 4MS+BA0.5mg / L; the medium for inducing rooting is ④1 / 4MS+IAA1mg / L+ PP3330.1mg / L

[0020] Breeding method:

[0021] 1. Establishment of sterile clones:

[0022] Material source: Plant hemp root (P.magen B.Q.Cheng ex C.L.long&J.Yang) is collected from the limestone wall at an altitude of about 1500m in Xishuangbanna area in southern Yunnan.

[0023] The establishment of the sterile line is firstly the disinfection and sterilization of the explants. The sterilization of the explant materials is an important link in the tissue culture,...

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PUM

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Abstract

The invention provides a rapid propagation method of a national spiceberry yarn (P.magen B.Q Cheng ex C.L.long&J.Yang). The method includes steps of selecting rapid propagation materials, optimizing culture bases and building up a sterile clonal culture, promoting formation of clustered buds, rooting and cultivating. The rapid propagation method is strong in operability, and able to rapidly enlarge the base number of sprouts, solve the problem of slow propagation of the rare (P.magen B.Q Cheng ex C.L.long&J.Yang), and provide technical guarantee for meeting the Dai nationality's compatriot favorite in the spice, protection and application, and for the species protection and introduction for regression in future.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a rapid propagation method of national spice plant hemp root ((P.magen B.Q.Chengex C.L.long&J.Yang)) technical background: [0002] Piper is the largest genus of the family Piperaceae, with about 1000-2000 species. This genus is mainly distributed in the tropics, and there are about 60 species in China (Cheng et al.1999), among which more than 30 species of Piper have good medicinal value, and are used in folk traditions for expelling wind and cold, promoting qi and blood circulation, Dispersing blood stasis and relieving pain, analgesic effect. [0003] Magen (P.magen B.Q.Cheng ex C.L.long&J.Yang) is a woody climbing vine that mainly grows on limestone walls at an altitude of about 1500m in Xishuangbanna area in southern Yunnan. The hemp root has a special fragrance and is used as a special food spice in the local minority communities. The Dai people in Banna almost bu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 罗吉凤杨珺王跃虎龙春林
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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