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Culture medium for elaeagnus angustifolia tissue culture for environment protection and method

A tissue culture and environmental protection technology, applied in the field of plant tissue culture, can solve the problems of no process and formula, etc., and achieve the effect of convenient and rapid reproduction

Inactive Publication Date: 2017-11-28
郭青华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There have been relevant literature reports on the tissue culture research of Elaeoptera, but there is no systematic and accurate industrial production process and formula

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A culture medium for the tissue culture of Elaeagnus sativa for environmental protection, including three kinds of primary culture medium, proliferation medium and rooting medium, wherein,

[0031] The first-generation medium formula: 1 / 2MS medium, kinetin 0.5mg / L, sucrose 25g / L, agar 3g / L; when preparing, first measure MS medium, add kinetin, add sucrose, after the sucrose dissolves, Adjust the pH to 5.8-6.0, add agar, heat to melt the agar, sterilize under high temperature and high pressure (121° C., 25 minutes), and cool to solid for later use.

[0032] Proliferation medium formula: MS medium, kinetin 1.0mg / L, indole acetic acid 0.5mg / L, sucrose 25g / L, agar 3g / L; when preparing, first measure MS medium, add kinetin, indole Acetic acid, add sucrose, after the sucrose is dissolved, adjust the pH to 5.8-6.0, add agar, heat to melt the agar, sterilize under high temperature and high pressure (121°C, 25 minutes), cool to a solid for later use;

[0033] Rooting medium for...

Embodiment 2

[0040] Different from Example 1, the first-generation buds in step 2 get lateral buds.

Embodiment 3

[0042] Different from embodiment two, the primary buds in step two get terminal buds.

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PUM

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Abstract

The invention discloses a culture medium for elaeagnus angustifolia tissue culture for environment protection. The culture medium comprises a primary culture medium, a proliferation culture medium and a rooting culture medium. The invention provides a culture medium formula which is particularly applicable to rapid proliferation of stem tip tissue of elaeagnus angustifolia; the primary culture medium is capable of promoting tissue growth and inducing lateral buds; the proliferation culture medium is capable of inducing buds rapidly for rapid propagation expansion; the rooting culture medium is applied to rooting culture; with elaeagnus angustifolia stem tip tissue for tissue culture, the purpose of rapid proliferation is achieved, and meanwhile the hereditary characters of elaeagnus angustifolia are maintained as much as possible; and due to synergic effects of the three culture mediums, rapid proliferation of the elaeagnus angustifolia stem tip tissue is facilitated, and a certain basis is made for later industrial production.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a medium for tissue culture of Elaeagnus erae for environmental protection. Background technique [0002] Elaeagnus, aliases: Qilixiang, Fragrant Willow, Thorny Willow, Osmanthus Willow, Silver Willow, Silver Willow Hudaozi, Yageda, Red Bean, Zegi Maodao, Gijiegedai, Latin name: Elaeagnus angustifolia Linn. Hu Deciduous trees or small trees of the genus Desperidaceae and Scorpio, 5-10 meters high, without thorns or with thorns, thorns 30-40 mm long, brown-red, shiny; young branches densely covered with silvery-white scales, old branches with scales Shedding, reddish brown, shiny. Leaf thin papery, oblong-lanceolate to linear-lanceolate, apex obtuse or obtuse, base cuneate, entire margin, with silvery-white round scales when young, partly shed when mature, greenish, below Off-white, densely covered with white scales, shiny, with inconspicuous lateral veins; petiole is slender...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 郭青华
Owner 郭青华
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