Method for nondestructive measurement of content of free hemoglobin in blood bag

Abstract

The invention discloses a method for nondestructive measurement of the content of free hemoglobin in a blood bag. The method comprises the following steps that a modulation apparatus modulates a light source; a displacement platform controls the light source to move to dual-light-path locations of a plurality of positions for transmission and excitation of blood separately; a spectrum receiving apparatus acquires the dual-light-path transmission and fluorescence spectra of each position; the dual-light-path transmission and fluorescence spectra are converted to frequency domains for construction of within-frequency-domain transmission spectra and within-frequency-domain fluorescence spectra; the logarithm of a ratio of transmission spectra in two frequency domains at each position is calculated so as to obtain the absorption spectrum of the blood at the position, the absorption spectra at the plurality of positions and the fluorescence spectra of the plurality of positions in dual-light-path frequency domains are subjected to normalization together, and then a mathematic model is established on the basis of the normalization and chemical examination data; and the absorption spectra and within-frequency-domain of an unknown complex solution at a plurality of positions are acquired by using same methods mentioned above, then subjected to normalization and finally substituted into the mathematic model so as to obtain the content of free hemoglobin.

Description

technical field [0001] The invention relates to the field of spectroscopic blood concentration analysis stoichiometry, in particular to a method for non-destructively measuring the content of free hemoglobin in a blood bag. Background technique [0002] In the existing technology, the relatively mature technology is to detect the content of free hemoglobin in the blood bag through chemical testing, which has the outstanding advantage of high accuracy, but the way of chemical testing cannot meet the needs of fast, non-contact, and pollution-free. Due to its non-contact and non-polluting characteristics, it is also possible to detect the content of free hemoglobin in blood bags. [0003] In spectral detection, according to the Lambert-Beer law: the incident light intensity I of each wavelength is measured separately 0 and the outgoing light intensity I, the absorbance A of each wavelength is calculated by the formula (1). ∈ is the absorption coefficient of the substance at a...

AI Technical Summary

Problems solved by technology

[0014] The invention provides a method for non-destructively measuring the content of free hemoglobin in a blood bag, the measurement is highly targeted, eliminates the influence of the spectral background noise, the change of the transmitted light source and the blood bag, suppresses the nonlinearity of the spectrum, and greatly improves the free hemoglobin content. The accuracy of content analysis solves the problem of non-destructive detection of free hemoglobin in blood bags, which is efficient and pollution-free. See the description below for details:

Images