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Biomarkers of Hypercoagulation State and Its Application

A state-marking and hypercoagulable technology, applied in the field of biomarkers of hypercoagulation state, can solve problems such as no research reports on urine biomarkers

Active Publication Date: 2019-05-14
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on biomarkers related to hypercoagulable state, currently available reports are mainly related to some blood biomarkers, but no relevant research reports on urine biomarkers

Method used

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  • Biomarkers of Hypercoagulation State and Its Application
  • Biomarkers of Hypercoagulation State and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, establishment of rat acute hypercoagulation state model

[0073] 1. Constructing an animal model of hypercoagulable state in rats based on phensulfame

[0074] 1. Experimental method

[0075] Twenty male wistar rats were randomly divided into two groups, 10 in each group, and weighed. One group of rats was intraperitoneally injected with phensulfame injection (250 mg / kg body weight), and was recorded as the experimental group; the other group of rats was injected with a corresponding volume of normal saline injection, and was recorded as the control group. The time of the first injection was recorded as 0h, and intraperitoneal injection was performed every 3h, for a total of 3 injections (that is, once at 0h, 3h and 6h). 9 hours after the first injection (i.e. 3 hours after the third injection), blood was collected from the inner canthus vein immediately, and the coagulation function of the rat was detected to determine the specifications for the formati...

Embodiment 2

[0090] Example 2. Urinary proteomics analysis and biomarker research in hypercoagulable state

[0091] 1. Experimental method

[0092] 1. Extraction of urine proteome by ethanol precipitation

[0093] Get the urine sample of the rat hypercoagulation state animal model based on different coagulation-promoting drugs (acetamine, tranexamic acid and aminocaproic acid) in Example 1 as the experimental group sample, and simultaneously use the control in each model The urine sample of the group was used as the control sample, and the protein group in the urine sample was extracted. The specific extraction method is as follows: Take 8 mL of urine sample in a clean 50 mL centrifuge tube, centrifuge: 2500 g, 30 min, 4 °C. Transfer the supernatant to a clean 50mL centrifuge tube, centrifuge: 12000g, 30min, 4°C. Measure the volume of the supernatant, transfer it to a clean 50mL centrifuge tube, add three times the volume of -20°C pre-cooled absolute ethanol, shake well, and settle at -20...

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Abstract

The invention discloses a biomarker having a high-blood coagulation status and an application of same. In the invention, three different blood coagulation promoting drugs (etamsylate, tranexamic acid and aminocaproic acid) are employed to respectively intervene in blood coagulation status of adult rats, thus successfully obtaining an adult rat acute high-blood coagulation status model; and then change on urine proteomes of the adult rats in intervention groups with different blood coagulation promoting drugs is analyzed; for the first time, the change feature of the urine proteomes under the high-blood coagulation status is systematically researched, and a biomarker, which is related to the high-blood coagulation status, is found in the urine. The invention initially discloses body intervention range and change factors of the high-blood coagulation status, which not only prompt related physiological and pathological mechanisms and the change feature of a signal pathway, but also are applied as an important biomarker in detection and / or diagnosis for related pathological change of the high-blood coagulation status or supply assistant information to the aspect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a biomarker of a hypercoagulation state and an application thereof. Background technique [0002] When the body is stimulated by physiological or pathological factors, it will produce a series of changes, and the changes that can be monitored are called biomarkers. The most fundamental characteristic of a biomarker is change. When the body is stimulated, the original balance in the body is broken, cells, tissues and organs will produce some abnormal metabolites and excrete them from the body to restore the original balance and maintain the homeostasis of the internal environment. Compared with blood, urine can accumulate a large number of changes because it is not regulated by the body's homeostasis mechanism, and can better reflect changes in the body, and is a good source of biomarkers. At the same time, urine has the advantages of large volume, non-invasiveness, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/86
CPCG01N33/86
Inventor 井健高友鹤杜震环秦伟伟
Owner BEIJING NORMAL UNIVERSITY
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