Molecular marker associated with colorectal carcinoma metastasis and prognosis as well as application of molecular marker
A colorectal cancer, application technology, applied in the field of tumor biodiagnosis and treatment, can solve the problem that there is no colorectal cancer metastasis molecular marker, etc.
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Embodiment 1
[0021] Example 1 Analyzing the mRNA expression level of RPL22L1 in clinical colorectal cancer samples through the database
[0022] The mRNA expression level of RPL22L1 in the expression profiling chip of clinical colorectal cancer samples from GEO and TCGA database was analyzed by Oncomine database. The result is as figure 1 shown, from figure 1 The results showed that the mRNA expression level of RPL22L1 in clinical colorectal cancer samples was significantly higher than that in paracancerous tissues.
Embodiment 2
[0023] The detection of embodiment 2 clinical sample
[0024] 1. Sample
[0025] 10 pairs of clinically matched colorectal cancer (1-10T) and adjacent (1-10N) tissues and pathological sections.
[0026] 2. Method
[0027] 2.1 Detection of RPL22L1 mRNA level in clinical colorectal cancer samples
[0028] 2.1.1 Extraction of RNA from colorectal cancer tissue
[0029] (1) Take a 2ml Eppendorf tube, add 100mg frozen tissue, 100μl 0.5mm zirconium silicate beads, 1ml Trizol.
[0030] (2) Put it in a tissue breaker, and break it at the maximum speed for 5 minutes.
[0031] (3) Add 0.2 mL of chloroform, shake vigorously for 30 s, and let stand on ice for 3 to 5 min.
[0032] (4) Centrifuge at 4°C, 12 000g, 15min.
[0033] (5) Transfer the upper aqueous phase to a new Eppendorf tube, add an equal volume of isopropanol to the supernatant, mix gently, and place at room temperature for 10 minutes.
[0034] (6) Centrifuge, 4°C, 12 000g, 15min.
[0035] (7) Discard the supernatant, ...
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