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A kind of standardized feeding method of Aedes albopictus larvae

A technology for Aedes albopictus and larvae, which is applied in the field of artificial breeding of Aedes albopictus, can solve the problems of unreachable larvae, difficulty in promotion, and reduced survival rate of larvae, so as to solve developmental asynchrony and ensure normal growth and survival rate. , the effect of improving the yield of male pupae

Active Publication Date: 2019-06-21
GUANGZHOU WOLBAKI BIOTECH CO LTD
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  • Claims
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AI Technical Summary

Problems solved by technology

Therefore, under the conditions of large-scale production in laboratories and workshops, it is of great significance to artificially raise mosquito larvae to provide standard experimental larvae for scientific experiments. However, there are few relevant research reports on the artificial breeding of mosquito larvae at home and abroad.
With the existing non-standardized artificial breeding method, the survival rate of larvae is significantly lower than that under natural environmental conditions, and the appearance of irregular development, etc., does not meet the standard larvae required for experiments or production. It is not suitable for large-scale breeding, and it is difficult to promote

Method used

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  • A kind of standardized feeding method of Aedes albopictus larvae
  • A kind of standardized feeding method of Aedes albopictus larvae
  • A kind of standardized feeding method of Aedes albopictus larvae

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Embodiment 1

[0017] A standardized breeding method for Aedes albopictus larvae, the method comprises the following steps: when the temperature is 24-28°C and the relative humidity is 60-90%, the hatched Aedes albopictus larvae are 3000-8000 per tray The stocking density was put into the feeding tray and raised. During the feeding process, the suspension feed was added quantitatively every day according to the stocking density, and the amount of the suspension feed was as shown in Table 1 within the feeding time of one week. The length of the feeding tray was 60cm. , 40cm in width and 6cm in height, put into 3-5L of clean water with a water depth of about 1cm-2cm; the suspension feed, in parts by mass, includes 60-84 parts of beef liver powder and 24-36 parts of shrimp powder. 12-36 parts of yeast powder, 2000 parts of water. Through standardized feeding, the survival rate of larvae can reach more than 95%; when the larvae become pupae for 45 hours, the pupae rate reaches 60%-80%; 1.15-1.3...

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Abstract

The invention discloses a standard breeding method of aedes albopictus larvae. The method comprises the steps as follows: 3,000-8,000 hatched aedes albopictus larvae are put in each breeding plate for breeding at the temperature of 24-28 DEG C and at the relative humidity of 60%-90%, during breeding, suspension feed is added quantitatively every day according to the breeding density, and the amount of the suspension feed is progressively decreased from the middle time period of the fourth and / or fifth day to time periods at two ends in the feeding time period of one week. The method creates conditions for male and female separation of aedes albopictus pupae, powerfully guarantees mass production in laboratories and factories and solves the problem of non-synchronous development in the aedes albopictus larva breeding process.

Description

[0001] Technical field: [0002] The invention relates to the technical field of artificial breeding of Aedes albopictus, in particular to a standardized breeding method for Aedes albopictus larvae. [0003] Background technique: [0004] Breeding insects artificially is one of the basic techniques for studying entomology. It can be used to breed certain types of insects without seasonal restrictions for research on insect biology, physiology, and formulation of integrated pest control measures. Mosquitoes (mosquito) belong to the family Insecta Diptera, and there are about 3000 species in the world. It is a tiny flying insect with piercing-sucking mouthparts. Usually the females feed on blood, while the males drink the sap of plants. Blood-sucking female mosquitoes are intermediate hosts for dengue, Zika, malaria, yellow fever, filariasis, Japanese encephalitis, and other pathogens. At present, with the continuous deepening of research on mosquito ecology and control techno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 王晓华朱俭张继昕尹光敏
Owner GUANGZHOU WOLBAKI BIOTECH CO LTD
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