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A kind of bacillus licheniformis and application thereof

A technology of Bacillus licheniformis and spore count, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of polluting the ecological environment of the vineyard, affecting the quality of grapes, and exceeding the standard of pesticide residues, achieving good control effect, no residue specificity, The effect of protecting the environment

Active Publication Date: 2020-04-28
HENGSHUI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the above problems that chemical pesticides are used in large quantities to prevent and control grape white rot in production, which seriously pollutes the ecological environment of the vineyard, causes pesticide residues to exceed the standard and affects the quality of grapes, etc. Compound composition and compound preparation of Bacillus licheniformis with good control effect

Method used

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  • A kind of bacillus licheniformis and application thereof
  • A kind of bacillus licheniformis and application thereof
  • A kind of bacillus licheniformis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The acquisition of embodiment 1 bacillus licheniformis (Bacillus licheniformis) Y24 bacterial strain

[0035] Samples were collected from grape leaves in a grape plantation in Zhaoquan Town, Hengshui City, Hebei Province, and brought back to the laboratory in sterile paper bags for isolation. Using the dilution plate separation method, grape leaves were chopped and suspended in sterile water containing 1% Tween 80, 100 μL of the suspension was spread on YEPD solid medium, cultured at 28°C for 3 days, and single colonies were picked for identification.

[0036] Bacillus licheniformis (Bacillus licheniformis) Y24 strain, the colony on NA medium is flat, with irregular edges, yellowish, rough and opaque surface, and often forms wrinkles when growing in liquid medium. Cell shape and arrangement are rod-shaped and solitary. Gram-positive, 2.5-3.5 μm long and 0.8-1.5 μm wide, producing mesophilic oval spores with slightly enlarged cysts. Capsular, flagella-less, motile. Th...

Embodiment 2

[0037] Embodiment 2 compound composition

[0038] The preparation method of the 2 billion bacillus licheniformis Y24 live spores / ml water preparation is prepared by adding 1% Tween 80 and 0.5% sorbitol to the fermented liquid with a spore content of 2 billion / ml.

[0039] Zhongshengmycin technical: produced by Shenzhen Nuopuxin Agrochemical Co., Ltd., the dosage form is wettable powder, and the content of Zhongshengmycin is 3%.

[0040] Mix 2 billion live spores of Bacillus licheniformis Y24 / ml with Zhongshengmycin technical product according to the mass percentage in Table 1.

[0041] Table 1 compound composition embodiment list

[0042]

Embodiment 3

[0043] Embodiment 3 compound preparation

[0044] Compound One

[0045] Take 2 billion Bacillus licheniformis Y24 live spores / ml water solution 100mL, 100g of Zhongshengmycin, add 95g of kaolin, 3g of sodium lignosulfonate, 2g of ammonium sulfate, 1g of polyethylene glycol, mix well and then dry, Pulverize and sieve with 320 mesh to obtain the compound preparation. Among them, the content of live spores of Bacillus licheniformis Y24 is 660 million / ml, the content of Zhongshengmycin is 1%, the content of kaolin is 31.4%, the content of sodium lignosulfonate is 1%, the content of ammonium sulfate is 0.6%, polyethylene glycol The alcohol content is 0.3%.

[0046] Compound preparation two

[0047] Take 2 billion Bacillus licheniformis Y24 live spores / ml water solution 100mL, 100g of Zhongshengmycin, add 100g of kaolin, 5g of sodium lignosulfonate, 4g of ammonium sulfate, 2g of polyethylene glycol, mix well and then dry, Pulverize and sieve with 320 mesh to obtain the compound ...

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Abstract

The invention provides a Bacillus licheniformis Y24 strain, which is preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC). The preservation date is September 25, 2015, and the preservation number is CGMCC No.11448. After the strain is compounded with Zhongshengmycin, it has a synergistic effect and can enhance the control effect on grape white rot. Through the use of compound compositions and compound preparations, a large number of beneficial microorganisms are introduced into the ecological environment of the vineyard, which deteriorates the living environment of pathogenic bacteria; combined with agricultural measures such as cultivation management, a stable, balanced and biologically diverse vineyard is formed Ecosystem, to achieve the purpose of one medicine, multiple cures, and lasting control of grape disease epidemics.

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a bacillus licheniformis compounded with Zhongshengmycin and its application in the control of grape white rot. Background technique [0002] In recent years, green food production has become the main direction of international agricultural product production. With the development of science and the deepening of human awareness of environmental protection and human health, green food production has attracted more and more attention from all over the world. Grape plays an important role in fruit trees all over the world, and it ranks second in terms of cultivated area and output. In China, grapes also have a long history of cultivation, according to historical records more than two thousand years. The biology and cultivation methods of grapes are special. Therefore, they are vulnerable to pathogens during their growth and development. The variety of diseases an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01N47/18A01P3/00C12R1/10
CPCA01N47/18A01N63/00C12N1/205C12R2001/10A01N2300/00
Inventor 马光郭继平
Owner HENGSHUI UNIVERSITY
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