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b Lymphoma and leukemia detection markers, kits and applications thereof

A technology for lymphoma and leukemia, applied in the field of medicine, can solve the problems of patients who are difficult to treat, have little effect, and are sensitive to chemotherapy drugs

Active Publication Date: 2021-07-23
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although more than 50% of patients can be cured by this treatment plan, at least 1 / 3 of patients are refractory to treatment or relapse
Such patients will be treated with autologous hematopoietic stem cell transplantation to sensitize them to chemotherapy drugs of the standard regimen, but with little success

Method used

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  • b Lymphoma and leukemia detection markers, kits and applications thereof
  • b Lymphoma and leukemia detection markers, kits and applications thereof
  • b Lymphoma and leukemia detection markers, kits and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. Immunohistochemical analysis of the expression of P4HA2 protein in diffuse large B lymphoma.

[0054] Test materials and their preparation: 205 cases of diffuse large B lymphoma and 20 cases of reactive hyperplastic lymph node tissue samples were collected and fixed in buffered formalin for 24 hours. Rinse with running water for 1 hour, place the sample in 30% and 50% ethanol for 30 minutes each, and finally store in 70% ethanol at 4°C. The fixed samples were first dehydrated with gradient ethanol, transparent in xylene, and then embedded in paraffin at about 52°C, and the slices with a thickness of 4-10 μm and a diameter of 0.1 mm were pasted on Polyurethane in a dot arrangement. Tissue chips were prepared on clean glass slides treated with amino acid. Bake the slices overnight at 34°C and store in a sealed container at 4°C.

[0055] Operation method: Take the prepared tissue chip, dewax it with xylene, rehydrate with gradient ethanol, then add 0.3% hydrog...

Embodiment 2

[0057] Example 2, the expression intensity of P4HA2 protein is related to the classification and prognosis of diffuse large B lymphoma

[0058]According to the results of Example 1, the correlation between the expression of P4HA2 protein in diffuse large B lymphoma and clinicopathological parameters was further analyzed. The results are shown in Table 1. There was no correlation between the expression of P4HA2 and age and gender, but there was a significant correlation with the classification and prognosis of diffuse large B lymphoma. P4HA2 expression was higher in patients with more aggressive non-GCB diffuse large B lymphoma. The IPI is scored by five pathological indicators related to prognosis, and the higher the score, the worse the prognosis. The higher the expression of P4HA2, the higher the IPI score, and the poorer the prognosis of diffuse large B lymphoma.

[0059] Table 1. Correlation analysis between P4HA2 expression intensity and clinicopathological indicators ...

Embodiment 3

[0061] Example 3, Analysis of the expression intensity of P4HA2 protein and the median survival time of patients with diffuse large B lymphoma

[0062] According to the results of Example 1, we also analyzed the correlation between P4HA2 and the median survival time of patients with diffuse large B lymphoma. It was found that the stronger the expression of P4HA2, the shorter the median survival time of patients with non-GCB diffuse large B lymphoma. This shows that P4HA2 is indeed closely related to the occurrence and development of diffuse large B lymphoma, and can be used as a marker for the diagnosis or treatment of diffuse large B lymphoma.

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Abstract

The invention belongs to the technical field of medicine, and specifically relates to a detection marker for B lymphoma and leukemia, a kit and an application thereof. The invention provides the use of P4HA2 gene or protein and Carabin protein in preparing diagnostic reagents or treating B lymphoma and leukemia. Studies have shown that P4HA2 gene and protein are almost non-expressed or low-expressed in normal or reactive hyperplastic lymph nodes, but significantly high-expressed in diffuse large B lymphoma; while Carabin protein is low-expressed in diffuse large B lymphoma. In addition, the expression level of P4HA2 gene in acute myeloid leukemia blood samples was significantly higher than that of normal people; the proliferation of diffuse large B lymphoma cell lines knocked down P4HA2 expression slowed down, and the tumorigenic ability of nude mice was significantly reduced; therefore, P4HA2 can As a diagnostic marker and therapeutic target for such hematopoietic and lymphoid tumors, Carabin can also be used as a diagnostic marker for such diseases. The invention also provides corresponding detection methods and kits.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a detection marker for B lymphoma or leukemia, a kit and its application in the preparation of antitumor drugs. Background technique [0002] Prolyl-4-hydroxylase (P4H) catalyzes proline residues in mammalian cells, yielding (2S,4R)-4-hydroxyproline. Prolyl-4-hydroxylase has a wide range of substrates, the most important of which is to catalyze the newly synthesized collagen precursor, and its folding to form a correct three-dimensional conformation is critical and necessary. The substrate for mammalian prolyl-4-hydroxylase is an alpha 2 beta 2 A tetramer whose α-subunit has a substrate-binding domain and an enzymatic active site. The α subunit has three subtypes, and the subunit type 1 (prolyl 4-hydroxylase, alpha polypeptide Ⅰ, referred to as "P4HA1") is the most widely expressed enzyme. Prolyl 4-hydroxylase type II α subunit (prolyl 4-hydroxylase, alpha polype...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N15/12C12N9/02C07K14/47C12Q1/6886G01N33/68G01N33/574G01N33/573
CPCC07K14/47C12N9/0071C12Q1/6886C12Q2600/156C12Q2600/158C12Y114/11002G01N33/573G01N33/57426G01N33/68G01N2333/90245
Inventor 党永军蒋维李增霞周晓燕刘凯玉崔照盟谭仁可
Owner FUDAN UNIV
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