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Application of Arabidopsis thaliana SAL gene on regulating flowering time and amount of rosette leaves

A technology of flowering time and Arabidopsis thaliana, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as lack of vegetative growth, low photosynthetic products, and small number of leaves

Inactive Publication Date: 2017-02-08
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, plants have been considered to have a trade-off between vegetative and reproductive growth, and therefore crop yields are considered to have an upper limit, because crops with early flowering are relatively deficient in basic vegetative growth (fewer leaves, photosynthetic products low volume) resulting in low yield

Method used

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  • Application of Arabidopsis thaliana SAL gene on regulating flowering time and amount of rosette leaves
  • Application of Arabidopsis thaliana SAL gene on regulating flowering time and amount of rosette leaves
  • Application of Arabidopsis thaliana SAL gene on regulating flowering time and amount of rosette leaves

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Experimental program
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Embodiment 1

[0014] 1. Obtaining Mutants

[0015] All SAL1 / 2 mutants used were ordered from the Arabidopsis Resource Center (ABRC, The Ohio State University Rightmire Hall 1060 Carmack Road Columbus, OH 43210 USA). in sal1 The number is SALK_052626, sal2-1 The serial number is SALK_018029c, sal2-2 The number is SALK_027501, and all mutants are constructed by T-DNA insertion.

[0016] double mutant sal1sal2-1 and sal1sal2-2 The construction is obtained by crossing single mutants with each other, and the specific operation method is: look for flower buds that are still tightly closed, but the stigma starts to protrude from the top of the flower. Using sterilized tweezers, remove any flower buds from a plant that are not emasculated. Remove all parts of the flower except the pistil with sterilized forceps, and pollinate immediately. Select the flower that has bloomed and remove the pistil with sterilized forceps. Gently touch the emasculated stigma with pollen. If the stigma elo...

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Abstract

The invention provides application of an Arabidopsis thaliana SAL gene on regulating flowering time and amount of rosette leaves. By analyzing phenotype of a loss-of-function mutant homozygous plant of SAL1 / 2, as for single mutants sal1 and sal2 as well as a double mutant sal1sal2, compared with a wild type Col-0, the phenomena that the flowering times are all shortened by about 7 days, the amounts of the rosette leaves are added by 2-3 leaves during flowering, and the growth speeds of stalks after flowering are rapider. An obvious difference occurs between a flowering phenotype of an SAL1 / 2 mutant and normal early blossoming phenotype, i.e. the amount of the rosette leaves is added, and report on the phenotype does not exist; and through phenotypes of the SAL2 mutant and an overexpression plant, the function can be speculated to be of limiting too fast growth of the plant, and a study result of the gene function has the guiding significance of breaking through a neck bottle of increasing the crop yield.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of Arabidopsis thaliana SAL gene in regulating flowering time and rosette leaf quantity. Background technique [0002] In flowering plants, the transition from vegetative to reproductive growth, anthesis, is the most critical stage of plant development. In agriculture, the timing of flowering largely determines the quantity and quality of seeds and fruits. The flowering of plants is affected by a series of environmental and endogenous factors, such as daylight length, circadian clock and gibberellin, etc., and the time of flowering is regulated by a complex gene regulatory network in vivo. Most of the genetic studies on plant flowering focus on the model plant Arabidopsis thaliana, and then apply the corresponding research results to other economic crops, such as rice, soybean and corn, which greatly improve the economic traits of crops and the adverse ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00
CPCC07K14/415C12N15/8261C12N15/827
Inventor 苏军冈义人黄志明
Owner FUJIAN AGRI & FORESTRY UNIV
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