Method for detecting concentration of vitamin B2 and vitamin B6 simultaneously

A vitamin and concentration technology, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problem of long analysis time and achieve the effect of accurate and rapid concentration detection

Active Publication Date: 2017-01-04
NANCHANG UNIV
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Problems solved by technology

Since vitamin B2 is a quencher of vitamin B6, the three-dimensional fluorescence method can only detect the mixed solution of vitamin B2 and vitamin B6 with a concentration upper limit of 2 μg / ml, and the analysis time of the three-dimensional fluorescence method is relatively long

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  • Method for detecting concentration of vitamin B2 and vitamin B6 simultaneously
  • Method for detecting concentration of vitamin B2 and vitamin B6 simultaneously
  • Method for detecting concentration of vitamin B2 and vitamin B6 simultaneously

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Embodiment Construction

[0014] The invention will be further illustrated by the following examples.

[0015] 1. Acquisition of initial data.

[0016] As shown in Table 1, vitamin B2 and vitamin B6 mixtures with different concentrations were prepared, and the fluorescence intensity was detected at Ex / Em 480 / 530nm and Ex / Em 300 / 400nm respectively. The concentration of vitamin B2 (X) was calculated from the vitamin B2 standard curve detected under the condition of Ex / Em 480 / 530nm. The apparent concentration (Y) of vitamin B6 was calculated from the vitamin B6 standard curve detected under the condition of Ex / Em 300 / 400nm. An equal volume of water was added as a control, and the actual concentration (Z) of vitamin B6 was calculated from the vitamin B6 standard curve detected under Ex / Em at 300 / 400nm.

[0017] Table 1 Apparent concentration (Y) and actual concentration (Z) of different concentrations of vitamin B2 (X), vitamin B6 (unit: μg / ml)

[0018] X Y Z X Y Z X Y Z 2.0 1.756 ...

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Abstract

The invention relates to a method for detecting the concentration of vitamin B2 and vitamin B6 simultaneously. The method comprises the following steps: measuring the fluorescence intensity of a mixed solution of the vitamin B2 and the vitamin B6 with different known concentrations; establishing a mathematic model which shows the fluorescence excitation influence of the vitamin B2 at different concentrations on the vitamin B6; quickly detecting the vitamin B2 and the vitamin B6 under a mixed system by using the model. The mixed liquid of the vitamin B2 with higher concentration and the vitamin B6 is detected by a fluorescence spectrophotometry method, and the influence rule of the vitamin B2 at different concentrations on the quenching efficiency of the vitamin B6 is explored, so that the concentration of the vitamin B2 and the concentration of the vitamin B6 can be detected accurately and quickly under the mixed system.

Description

technical field [0001] The invention belongs to the field of food testing, and mainly relates to the detection of the concentrations of vitamin B2 and vitamin B6 in a mixed system. Background technique [0002] The detection of vitamin B2 and vitamin B6 content in the mixed system generally adopts the HPLC method, because this method can better realize the separation and detection of vitamin B2 and vitamin B6 in the mixed system. However, the reagents required by this method need to be strictly purified, the analysis time is long, and the detection cost is high. [0003] Another approach is to use three-dimensional fluorescence method for detection and quantification. Since vitamin B2 is a quencher of vitamin B6, the three-dimensional fluorescence method can only detect the mixed solution of vitamin B2 and vitamin B6 with a concentration upper limit of 2 μg / ml, and the analysis time of the three-dimensional fluorescence method is relatively long. Contents of the invention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 吴志华李晓飞陈红兵李欣佟平杨安树
Owner NANCHANG UNIV
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