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Application of TLR3 in predicting tumor transfer, evaluating prognosis and selecting prevention and treatment schemes

A technology of tumor metastasis and tumor, applied in the fields of biotechnology and medical diagnosis

Active Publication Date: 2016-12-07
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] At present, there are no research reports on the correlation between host pattern recognition receptor TLR3 and neutrophil migration, tumor metastasis and prognosis at home and abroad.

Method used

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  • Application of TLR3 in predicting tumor transfer, evaluating prognosis and selecting prevention and treatment schemes
  • Application of TLR3 in predicting tumor transfer, evaluating prognosis and selecting prevention and treatment schemes
  • Application of TLR3 in predicting tumor transfer, evaluating prognosis and selecting prevention and treatment schemes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: the preparation of detection kit

[0086]The detection kit is prepared according to the following composition, and the kit is suitable for detecting the expression of TLR3 in biological samples by immunohistochemical method:

[0087] Reagent a: blocking solution, 10% goat serum, 1 bottle, ready-to-use;

[0088] Reagent b: anti-human or mouse TLR3 monoclonal antibody (purchased from abcam company in the United States, catalog number ab13915), one, 200×concentrated solution;

[0089] Reagent c: anti-mouse biotinylated secondary antibody (purchased from Beijing Zhongshan Jinqiao Company, catalog number SP-9002), 1 bottle, ready-to-use;

[0090] Reagent d: HRP-labeled streptavidin, 1 bottle, ready-to-use;

[0091] Reagent e: DAB substrate buffer, one, 20× concentrated solution;

[0092] Reagent f: DAB chromogenic solution, one, 20× concentrated solution;

[0093] As well as containers containing the above reagents and instructions for use.

Embodiment 2

[0094] Example 2: Correlation between TLR3 expression in lung cancer paracancerous tissue and clinical factors of lung cancer patients

[0095] A total of 90 patients with lung cancer were selected in 2004 (tissue sections were from Shanghai Xinchao Biotechnology Co., Ltd.). The tissue types of their lung cancer were all non-small cell lung cancer (NSCLC). The patients did not receive chemotherapy and radiotherapy before surgery.

[0096] Using the immunohistochemical method, the TLR3 protein in the paracancerous tissues of 90 cases of lung cancer was stained using the kit in Example 1, and the correlation between the expression of TLR3 and the clinical factors of lung cancer patients was analyzed. P values ​​use Chi-square tests in SPSS 17.0 (Table 1). The results showed that the expression of TLR3 was correlated with the TNM stage of tumor patients (P value<0.05), that is, the higher the expression of TLR3 in the paracancerous tissue, the higher the TNM grade of the tumor. ...

Embodiment 3

[0104] Example 3: Correlation analysis between the expression of TLR3 molecules in lung cancer paracancerous tissues and tumor metastasis-related factors (S100A8 and S100A9)

[0105] To this 90 cases of lung cancer patients, adopt immunohistochemical method, utilize the test kit among the embodiment 1 to this 90 cases of TLR3 protein and tumor metastasis-related factors (S100A8 and S100A9, its detection reagent is the same as embodiment 1, Except that reagent b was replaced by S100A8 antibody, catalog number ab80708 or S100A9 antibody, catalog number ab24111 (both purchased from abcam, USA)) were stained to analyze the correlation between the expression of TLR3 and the expression of tumor metastasis-related factors (S100A8 and S100A9).

[0106] TLR3, S100A8 and S100A9 immunohistochemical score: The expression of TLR3, S100A8 and S100A9 protein was scored by quick score (QS). First, the proportion of positive cells is scored according to 1-6 points (1=1-4%; 2=5-19%; 3=20-39%; 4...

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Abstract

The invention relates to application of TLR3 in predicting tumor transfer, evaluating prognosis and selecting prevention and treatment schemes. The invention particularly relates to application of a substance for detecting a pattern recognition receptor TLR3 in a biological sample in preparing products for predicting tumor transfer in the object and / or evaluating object tumor prognosis and / or selecting tumor prevention and treatment schemes, and a corresponding product and method thereof. The invention provides application of the pattern recognition receptor TLR3 in predicting tumor transfer, evaluating prognosis and selecting prevention and treatment schemes, also provides a corresponding product and method, and has certain clinical application prospects.

Description

technical field [0001] The invention relates to the fields of biotechnology and medical diagnosis, and in particular to the application of pattern recognition receptor TLR3 in tumor metastasis prediction, prognosis evaluation and prevention and control scheme selection. Background technique [0002] Tumor is a major disease that endangers human health, and tumor metastasis is the main cause of death in cancer patients. Tumor metastasis is the formation of new tumor clones when tumor cells travel from the primary site to other sites via lymphatic channels, blood vessels, or body cavities. Current studies have found that tumor metastasis is not only related to the tumor cells themselves, but also closely related to the microenvironment in which the tumor is located. [0003] The tumor microenvironment is composed of tumor cells and tumor-associated cells (mainly host cells), including vascular endothelial cells, fibroblasts, pericytes, and some inflammatory cells (Hanahan, D....

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68G01N33/574
CPCC12Q1/6886C12Q2600/106C12Q2600/112C12Q2600/118G01N33/57492G01N33/6893G01N2333/705
Inventor 曹雪涛刘艳芳顾炎
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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