Preparing method for MC3R gene knockout pigs
A gene knockout and gene technology, applied in biochemical equipment and methods, genetic engineering, animal husbandry, etc., can solve problems such as low efficiency, long cycle, and troublesome mark gene deletion
Inactive Publication Date: 2016-12-07
CHINA AGRI UNIV
View PDF1 Cites 38 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
The construction of this targeting carrier is cumbersome, the cycle is long, the cost is high, and the efficiency is extremely low
And the deletion of mark gene is a troublesome problem, even after mark-free, the gene fragment of loxp or flt will be left
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0062] Example 1. Preparation of MC3R knockout cell lines and MC3R knockout pigs using CRISPR / Cas9 technology
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More
Abstract
The invention discloses a preparing method for MC3R gene knockout pigs. The preparing method for MC3R gene knockout pigs includes the steps that a CRISPR / Cas9 system is adopted, the CRISPR / Cas9 system comprises an encoding gene of gRNA1 and an encoding gene of gRNA2, and the target sequence of a MC3R gene identified by the gRNA1 is a sequence 1 in a sequence table; the target sequence of the MC3R gene identified by the gRNA2 is a sequence 2 in a sequence table. An experiment shows that the MC3R gene knockout efficiency is 29.16% with the preparing method for MC3R gene knockout pigs; a target gene can be rapidly and efficiently knocked out in a large-fragment mode, exogenous gene fragments are not left, and the preparing method can be used for researching the action mode and mechanism of the MC3R gene, and can be further used for animal breeding.
Description
technical field [0001] The invention relates to a preparation method of MC3R gene knockout pigs in the field of biotechnology. Background technique [0002] Melanocortin receptors (MCRs) are receptors located on the surface of mammalian cells, belonging to the G-protein coupled receptor family, and five members have been discovered so far. Under the stimulation of Melanocortin (MC), MCRs increase the concentration of the intracellular second messenger cAMP by binding to the activated Gs protein, and start the intracellular signaling pathway. The physiological role of each member of MCRs is very different. MC1R is expressed by skin melanocytes and plays a key role in determining skin and hair pigmentation. MC2R is expressed in the adrenal cortex and regulates adrenal steroid synthesis and cell proliferation. MC5R is widely distributed and may be related to exocrine function. MC3R and MC4R are believed to be involved in the regulation of energy balance in the body. In rece...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85A01K67/027
CPCA01K2267/03C07K14/723C12N15/8509
Inventor 李秋艳徐邢宾郝海阳张瑾李志远罗洁
Owner CHINA AGRI UNIV
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com