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Preparation of soybean protein chitosan microspheres combined with amino acid metal complexes and its application as an antioxidant

A technology of metal complexes and soybean protein, applied in the field of SOD enzyme mimics, to achieve the effect of improving the ability to scavenge oxygen free radicals

Active Publication Date: 2018-04-06
SHENYANG DEKANG MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But few people combine it with amino acid Schiff base metal complexes as SOD enzyme mimics

Method used

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  • Preparation of soybean protein chitosan microspheres combined with amino acid metal complexes and its application as an antioxidant
  • Preparation of soybean protein chitosan microspheres combined with amino acid metal complexes and its application as an antioxidant
  • Preparation of soybean protein chitosan microspheres combined with amino acid metal complexes and its application as an antioxidant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Dissolve 0.05 g of chitosan in 50 mL of 0.1 mol / L hydrochloric acid solution with stirring for 0.5 h to obtain an acidic chitosan solution; disperse 0.08 g of soybean protein in 20 mL of 10 mol / L urea solution and stir for 1 h , so that the soybean protein was completely dissolved to obtain a soybean protein dispersion; then at room temperature and under high-speed stirring, slowly drop 5 mL of the soybean protein dispersion into 50 mL of acidic chitosan solution, and after stirring for 30 min, use a 18,000 Da dialysis bag Dialyze for 4 h to obtain soybean protein-chitosan nanosphere dispersion.

[0039]Dissolve 2 g of amino acid Schiff base metal complexes in 5 mL of ethanol, take 0.15 mL of this solution, add it to 2 mL of soybean protein-chitosan nano-microsphere dispersion, stir at room temperature for 1 h in the dark, and then use The 18000 Da dialysis bag was dialyzed for 1 h to obtain the complex complexes of amino acid Schiff base metal complexes of soybean prot...

Embodiment 2

[0041] Disperse 0.02 g of soybean protein in 10 mL of 1 mol / L NaOH solution and stir for 1 h to completely dissolve the soybean protein to obtain a soybean protein dispersion; dissolve 0.2 g of chitosan in 100 mL of 0.5 mol / L hydrochloric acid The solution was stirred and dissolved for 0.5 h to obtain an acidic chitosan solution. At room temperature and under high-speed stirring, 10 mL of soybean protein dispersion was slowly dropped into 80 mL of acidic chitosan solution, stirred for 60 min, and dialyzed for 4 h with a 15,000 Da dialysis bag to obtain soybean protein-chitosan nanosphere dispersions. liquid.

[0042] Dissolve 10 g of amino acid Schiff base metal complexes in 30 mL of dimethyl sulfoxide solvent, take 0.15 mL of the solution, add it to 2 mL of soybean protein-chitosan nano-microsphere dispersion, and stir at room temperature in the dark 1 h, and then dialyzed with 15000 Da dialysis bag for 1 h to obtain soybean protein-chitosan nano-microsphere amino acid Schif...

Embodiment 3

[0044] Disperse 0.02 g soybean protein in 15 mL of 0.5 mol / L KOH solution and stir for 3 h to completely dissolve the soybean protein to obtain a soybean protein dispersion; dissolve 0.2 g chitosan in 80 ml 0.3 mol / L acetic acid The solution was stirred and dissolved for 0.5 h to obtain an acidic chitosan solution. At room temperature and under high-speed stirring, slowly drop 15 mL of soybean protein dispersion into 10 mL of acidic chitosan solution, continue stirring for 10 min, and dialyze for 4 h with a 12,000 Da dialysis bag to obtain soybean protein-chitosan nanospheres. Dispersions.

[0045] Dissolve 10 g of amino acid Schiff base metal complex in 30 mL of ethanol solvent, take 0.25 mL of the solution, add it to 8 mL of soybean protein-chitosan nano-microsphere dispersion, stir at room temperature for 1 h in the dark, Then, it was dialyzed for 2 h with a 12000 Da dialysis bag to obtain the soybean protein-chitosan nano-microsphere amino acid Schiff base metal complex c...

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Abstract

The invention provides a preparation method of a compound obtained through combining soybean protein chitosan microspheres with an amino acid schiff alkali metal complex. Two different types of natural macromolecules, i.e., soybean protein and chitosan are combined through hydrogen bond and hydrophobic effects to obtain natural macromolecule nanometer microspheres; then, the natural macromolecule nanometer microspheres are combined with an amino acid Schiff alkali metal complex to be prepared into a compound of the nanometer microspheres and the metal complex; the water solubility of the amino acid Schiff alkali metal complex is improved; meanwhile, the toxicity is reduced. The antioxygenic property study shows that the compound has high oxygen radical clearing capability, so that the compound can be used as an SOD (superoxide dismutase) enzyme mimic; good application prospects are realized in the aspect of preparing antioxidant medicines.

Description

technical field [0001] The present invention relates to a preparation method of a soybean protein chitosan nano-microsphere amino acid metal complex composite material. The present invention also relates to the application of the composite material as a SOD enzyme simulant in the preparation of antioxidant drugs, which belongs to natural macromolecule Composite materials and biomedicine. Background technique [0002] Reactive oxygen species include superoxide anion radicals, hydroxyl radicals, hydrogen peroxide, and singlet oxygen, which are natural byproducts of human metabolism. However, when it is in excess, it attacks biomolecules such as lipids, proteins, enzymes, DNA and RNA, causing cell or tissue damage. Superoxide dismutase (SOD) is a metalloenzyme in vivo, which can catalyze the disproportionation reaction of oxygen free radicals and balance oxygen free radicals in the body. Although natural SOD has a strong ability to scavenge oxygen free radicals, its extractio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L89/00C08L5/08C08K5/00A61P39/06
CPCC08L5/08C08L89/00C08L2201/08C08L2201/10C08K5/0091
Inventor 何玉凤闫桂芳王荣民徐知丽王建风
Owner SHENYANG DEKANG MEDICINE TECH CO LTD
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