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A method for detoxification and rapid propagation of kiwifruit stem tips

A technique for detoxification of stem tips and kiwifruit, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that do not involve detoxification of kiwifruit stem tips, short internode distance, low reproduction coefficient, etc. The effect of surviving after planting, increasing internode distance and increasing reproduction coefficient

Active Publication Date: 2018-01-16
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are still some defects in the existing stem tip detoxification technology, such as short internode distance, low reproduction coefficient, low survival rate, etc., which cannot meet the needs of rapid reproduction, and there is no related technology related to kiwifruit stem tip detoxification. technology

Method used

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  • A method for detoxification and rapid propagation of kiwifruit stem tips

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Experimental program
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Effect test

Embodiment 1

[0022] (1) material selection: screen strong Hongyang kiwifruit plants from the kiwifruit orchard in March, mark them well, cut the 1-2cm long terminal buds, and set them aside;

[0023] (2) Disinfection of materials: first rinse with running water under tap water for 2 hours, cut off the fully expanded leaves, then soak them in 75% ethanol for 20 seconds under a sterile operating table, rinse them with sterile water, and then use 0.1% HgCl 2 Soak for 5 minutes, then rinse 4 times with sterile water;

[0024] (3) Peel off the shoot tip: use the shoot tip with 1-2 leaf primordia aseptically stripped from the terminal bud obtained in (2) under a dissecting microscope as an explant, the shoot tip size is 0.15-0.3mm, and the shape is a protrusion State, put the shoot tip into the medium (MS+6-BA1mg / L+GA 3 0.1mg / L, agar 6g / L, sucrose 25g / L, pH5.8) for inoculation and culture, the culture conditions are temperature 25-27°C, light intensity 1500Lux, and the number of light hours per...

Embodiment 2

[0028] (1) material selection: screen strong Hongyang kiwifruit plants from the kiwifruit orchard in March, mark them well, cut the 1-2cm long terminal buds, and set them aside;

[0029] (2) Material disinfection: first rinse with running water under tap water for 2 hours, cut off the fully expanded leaves, then soak them in 75% ethanol for 30 seconds under a sterile operating table, rinse them with sterile water, and then use 0.1% HgCl 2 Soak for 6 minutes, then rinse 6 times with sterile water;

[0030] (3) Peel off the shoot tip: use the shoot tip with 1-2 leaf primordia aseptically stripped from the terminal bud obtained in (2) under a dissecting microscope as an explant, the shoot tip size is 0.15-0.3mm, and the shape is a protrusion State, put the shoot tip into the culture medium (MS+6-BA3mg / L+GA 3 0.3mg / L, agar 6g / L, sucrose 25g / L, pH5.8) for inoculation culture, the culture conditions are temperature 25-27 ℃, light intensity is 2000Lux, and the number of light hours ...

Embodiment 3

[0034] (1) material selection: screen strong Hongyang kiwifruit plants from the kiwifruit orchard in March, mark them well, cut the 1-2cm long terminal buds, and set them aside;

[0035] (2) Material disinfection: first rinse with running water under tap water for 2 hours, cut off the fully expanded leaves, then soak them in 75% ethanol for 25 seconds under a sterile operating table, rinse them with sterile water, and then use 0.1% HgCl 2 Soak for 5.5min, then rinse 5 times with sterile water;

[0036] (3) Peel off the shoot tip: use the shoot tip with 1-2 leaf primordia aseptically stripped from the terminal bud obtained in (2) under a dissecting microscope as an explant, the shoot tip size is 0.15-0.3mm, and the shape is a protrusion State, put the shoot tip into the culture medium (MS+6-BA2mg / L+GA 3 0.2mg / L, agar 6g / L, sucrose 25g / L, pH 5.8) for inoculation and culture, the culture conditions are temperature 25-27°C, light intensity 1800Lux, and the number of light hours p...

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Abstract

The invention discloses a Chinese gooseberry stem tip detoxification and rapid propagation method. The method comprises the following steps of 1 material selecting, 2 material disinfecting, 3 stem tip stripping, 4 strain building and virus detecting and 5 rapid propagating. Compared with the prior art, the Chinese gooseberry stem tip detoxification and rapid propagation method has the advantages that viruses carried by plants can be effectively removed, and therefore a large quantity of virus-free plants are cultured; the Chinese gooseberry internode distance can be significantly increased, therefore, the propagation coefficient and the survival rate after transplanting are increased, and the rapid propagation requirement of Chinese gooseberry is greatly met.

Description

technical field [0001] The invention relates to a method for detoxification and rapid propagation of kiwifruit stem tips, which belongs to the field of nursery stock breeding. Background technique [0002] Actinidiaceae (Actinidiaceae) is a perennial vine of the genus Actinidia. It is one of the four earliest wild fruit trees domesticated and cultivated in the 20th century. Its fruit has rich nutritional value and good medicinal value, especially containing a large amount of Vc , enjoying the title of "King of Vc". Hongyang kiwifruit belongs to the Chinese series of kiwifruit. It is a rare red heart kiwifruit in the kiwifruit family. It is a natural variation variety found in Cangxi, the origin of kiwifruit in Sichuan. It has high nutritional value, and the flesh is tender and sweet, with early fruit and high yield Strong in nature and stress resistance, it is very popular in the market and has a very high promotion prospect. [0003] With the development of kiwifruit indu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王姗鲍华鹏鲍荣静雷武生王全智
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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