revascularization graft material
A graft material and a technique for vascular reconstruction, which are applied in the fields of blood vessels, devices for human tubular structures, medical science, etc., and can solve the problem of not describing multi-tubular structures.
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example 2
[0149]
[0150] The hollow tubular structure obtained in Reference Example 1 was immersed in adeno-associated virus (AAV) (phosphate buffered saline, virus concentration: 1×10 2 virus particles / ml), allowed to react at 37°C for 1 hour, and then washed with phosphate buffered saline. The 293 cells were cultured throughout the dish, the AAV-bound hollow tubular structure was placed on the cultured 293 cells, and the culture was continued for 3 weeks.
[0151] Such as Figure 5-1 As shown, only cells in contact with the hollow tubular structure emit green fluorescence. This shows that AAV is hardly released from the hollow tubular structure, and AAV in a state where activity is maintained is bound to the hollow tubular structure. Figure 5-2 is schematically shown in Figure 5-1 Diagram of the area in which the hollow tubular structure and the GFP-expressing cells in contact with the hollow tubular structure are located in the photographs shown.
example 1
[0152]
[0153] The hollow tubular structure having a single tube structure obtained from Reference Example 1 (hereinafter, in this example and in Examples 2 and 3, simply referred to as "the hollow tubular structure"), having a lumen diameter of 500 μm The hollow tubular structures and the hollow tubular structures having a lumen diameter of 100 μm were implanted by suturing these hollow tubular structures into the subcutaneous tissue of the lower limbs of rats. After 3 days, the site with the hollow tubular structure sutured therein was sampled, and histological sections were prepared according to a conventional method, and observed with an optical microscope. These results thus obtained are shown in Figure 6-1 (A) and Figure 6-1 (B).
[0154] Furthermore, in the hollow tubular structure obtained from Reference Example 1, the hollow tubular structure having a lumen diameter of 100 μm was implanted by suturing the hollow tubular structure into the muscle of the lower li...
example 3
[0163]
[0164] Then, for the purpose of verifying the fastening of the inner luminal space of the revascularization graft material, and at the same time to demonstrate the revascularization in the interior of the revascularization graft material, experiments were carried out in another strain of mice. A lower limb ischemia model was prepared in the same manner as in Example 2 by using female C57BL / 6J mice (from Charles River Corporation), and implanted with VEGF-bound hollow tubular structures or VEGF-bound vascular reconstruction grafts.
[0165] 3 weeks after implantation, 100 μl of biotin-conjugated tomato lectin (from Vector Laboratories) (with a content of 1 mg / ml) was injected into the tail vein; Intraperitoneal administration; 10 minutes after injection, a thoracotomy was performed, perfused with 20 ml of phosphate-buffered saline, and additionally with 2% paraformaldehyde solution.
[0166] The site where the hollow tubular structure was sutured, and the site where ...
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