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Preservation method of chlorella concentrate

A preservation method, the technology of chlorella, applied in the field of marine biology, can solve the problems of inconvenience in aquaculture industry, lack of preservation method of chlorella concentrate, etc., and achieve the effects of low cost, good preservation effect and wide application range

Inactive Publication Date: 2016-08-10
DONGTAI CITY SPIRULINA BIO ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Marine microalgae are rich in nutrients and contain a large amount of protein, polysaccharides, unsaturated fatty acids, multivitamins and other nutrients, and are widely used in aquaculture. However, the growth of microalgae is easily affected by environmental, climate and other factors, and often It brings great inconvenience to the aquaculture industry, so the research and development of the application of cryobiology in microalgae is getting more and more attention
[0006] At present, there is a lack of a preservation method for the chlorella concentrate with good preservation effect

Method used

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  • Preservation method of chlorella concentrate
  • Preservation method of chlorella concentrate
  • Preservation method of chlorella concentrate

Examples

Experimental program
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Effect test

Embodiment 1

[0017] A kind of preservation method of chlorella concentrate of the present invention comprises the following steps:

[0018] (1) Use 120mg / L of alum and 30mg / L of ferric chloride to flocculate the chlorella algae liquid respectively, centrifuge and concentrate the chlorella, prepare a chlorella concentrate and store it at 4°C for later use;

[0019] (2) Divide the prepared chlorella concentrate into 5ml cryopreservation tubes, then seal the cryopreservation tubes containing the chlorella concentrate with black plastic bags and place them in refrigerators at 4°C for storage. The time is 3 months. Example 2

Embodiment 2

[0020] The difference between embodiment 2 and embodiment 1 is: the preservation method of a kind of chlorella concentrate of the present invention, comprises the following steps: in step (1), use the alum of 180mg / L, the chlorination of 40mg / L The chlorella liquid is flocculated with iron, the chlorella is concentrated by centrifugation, and the chlorella concentrate is prepared and stored at 4°C for later use.

Embodiment 3

[0022] The difference between embodiment 3 and embodiment 1 is: a kind of preservation method of chlorella concentrate of the present invention comprises the following steps: in step (1), respectively use the alum of 200mg / L, the chlorination of 60mg / L Iron flocculation of chlorella algae liquid, centrifugal concentration of chlorella, prepared into chlorella concentrate and stored at 4°C for later use;

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Abstract

The invention discloses a preservation method of chlorella concentrate, comprising the following steps: (1), flocculating chlorella liquid respectively with 120-200 mg / L of alums and 30-60 mg / L of iron chloride, centrifuging and concentrating chlorella to prepare chlorella concentrate, and storing at 4 DEG C for use; (2), subpackaging the prepared chlorella concentrate in 5ml freeze-storing tubes, sealing the free-storing tubes where the chlorella concentrate is loaded, with black plastic bags, placing in a refrigerator at 4 DEG C, and storing for 3 months. The preservation method is simple to perform, good in preserving effect, widely applicable and low in cost.

Description

technical field [0001] The invention relates to the technical field of marine organisms, in particular to a method for preserving a chlorella concentrate. Background technique [0002] Microalgae are single-celled eukaryotic organisms with high photosynthetic utilization rate. The size is generally between 5 and 25 μm. It is impossible to observe its shape, structure, size and activity mode directly with the naked eye. It needs to be distinguished through a microscope. Cell shape, structure, etc., hence the name microalgae. It is widely distributed in various temperature zones, and there are microalgae from fresh water to sea water. Because of its strong photosynthesis, fast proliferation, easy artificial cultivation, and rich nutrition, microalgae are now widely used in aquaculture, drug research and development, sewage purification, food industry, pigment extraction, and energy development. Most microalgae are rich in polyunsaturated fatty acids such as eicosapentaenoic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/12C12R1/89
CPCC12N1/04C12N1/12
Inventor 高志刚汪世军王峰
Owner DONGTAI CITY SPIRULINA BIO ENG CO LTD
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