The method of using snp to promote the complete abortion of the pollen of thermosensitive genic male sterile wheat bns male sterile line

Abstract

The invention discloses a method for promoting complete abortion of pollen of the sterile line of temperature-sensitive genic male sterility wheat BNS. The method includes the steps that the temperature-sensitive genic male sterility wheat BNS is seeded under the sterility-period seeding condition to form the sterile line; from the time stamens grow to be in the pistil and stamen primordium differentiation period to the time wheat ears just completely exist from flag leaf sheaths, from the first day, BNS plants are subjected to whole-plant spraying with a sodium nitroprusside solution with the molar concentration of 200 micro-mol / L every four days, wherein the spraying amount is 50 L per mu to 80 L per mu; other steps refer to the common field cultivation management mode. According to the method, as field cultivation is adopted and the SNP solution with the molar concentration of 200 micro-mol / L is adopted for treatment, the abortion rate of the pollen of the sterile line of the BNS is at the complete abortion level, the self-crossing fruiting rate is 0%, the requirement for the sterile line during hybrid wheat production is met, operation is simple, investment cost is low, time and labor are saved, and broad application prospects are achieved.

Description

technical field [0001] The invention belongs to the technical field of chemical regulation and control of BNS pollen fertility of thermosensitive genic male sterile wheat, and in particular relates to a method for promoting complete abortion of pollen of thermosensitive genic male sterile wheat BNS male sterile line by using SNP. Background technique [0002] The utilization of wheat photothermo-sensitive male sterility (PTSMS) is a major advance in the research of hybrid wheat. The wheat light-temperature-sensitive sterile lines discovered in my country, such as ES, C49S, ZP, BS, YS and BNS, have become important test materials for hybrid wheat research. [0003] Thermo-sensitive genic male sterile wheat BNS was successfully bred by Ru Zhengang in 2002. BNS has the characteristics of complete sterility and complete fertility transformation. The changing trend of fertility is: complete abortion-semi-sterility-complete fertility. Low temperature is the main reason for affect...

AI Technical Summary

Problems solved by technology

Previous studies have pointed out that the existing chemical andricides such as ethephon can effectively kill males, but after the use of ethephon, there are many problems such as slowing down the development of wheat flowers, difficulty in heading, easy to green and late maturity, and easy to be infected with diseases and insect pests. Other existing chemical andricides are highly toxic to wheat plants, reduce male fertility, and have a strong inhibitory effect on pistil fertility. After artificial assisted pollination, the seed setting rate is low and the grains are not full, and the drug price is high. Therefore, the application of existing chemical andricides in production is limited, and existing chemical hybridization agents are used to overcome the interference of periodic fluctuations in temperature during the sensitive period of fertility conversion on the fertility stability of BNS male sterile lines of thermosensitive genic male sterile wheat At present, there are few reports, and the research on the use of sodium nitroprusside (SNP) spraying to promote the complete abortion of wheat BNS male sterile line pollen has not been reported yet.

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