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Root specific expression AhMtan promoter and application thereof

A root-specific, promoter technology, applied in applications, recombinant DNA technology, angiosperms/flowering plants, etc., can solve the problem of rare peanut root-specific promoter reports

Active Publication Date: 2016-06-22
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many root-specific promoters have been cloned, and their sources include tobacco, rice, corn, Arabidopsis, tomato, soybean, etc., but reports of peanut root-specific promoters are relatively rare

Method used

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  • Root specific expression AhMtan promoter and application thereof
  • Root specific expression AhMtan promoter and application thereof
  • Root specific expression AhMtan promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The present invention will be further described in detail below in conjunction with the embodiments.

[0039] 1. Experimental materials

[0040] 1.1 Plant material

[0041] Cultivated peanut (Arachishypogaea L.) variety Baisha No. 1016 was provided by Shandong Peanut Research Institute.

[0042] Tobacco (Nicotianabenthamiana), preserved by our laboratory.

[0043] 1.2 Strains and plasmids

[0044] The strains and plasmids used in this study are shown in Table 1. The following strains and plasmids can be distributed to the public.

[0045] Table 1 Strains and plasmids

[0046]

[0047] 1.3 Database and biological software

[0048] 1.3.1 Database

[0049] NCBI: http: / / www.ncbi.nlm.nih.gov /

[0050] 1.3.2 Biological software

[0051] DNAMAN6.0: sequence alignment analysis software

[0052] Primer5.0: Primer design software

[0053] 1.4 Reagent

[0054] 1.4.1 RNA extraction, reverse transcription and fluorescent quantitative PCR reagents

[0055] TotalRNAExtractor for RNA extraction was purcha...

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Abstract

The invention relates to a root specific expression AhMtan promoter and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the root specific expression AhMtan promoter is shown as SEQ ID NO2 or SEQ ID NO3 or SEQ ID NO4.Peanut root specific promoters are cloned, core areas of the promoters are determined through a truncation experiment, the functions of all the promoters are verified, and a foundation is laid for application and development of the peanut biotechnology of China. An important molecular basis is provided for providing the efficient, stable and special expression promoter for obtaining a bivalent grub resisting peanut variety.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a root-specific expression promoter, its cloning, functional verification and its application. Background technique [0002] Cultivated peanuts (Arachishypogaea L.) belong to legume crops and are important oil crops and economic crops in the world. In 2015, my country's peanut production reached 16.7 million tons, ranking first in the world. The yield of peanuts is affected by some biotic and abiotic stresses, especially underground pests, fungi, bacteria, etc. In recent years, the occurrence of underground pests in peanut fields has been rampant, which has seriously affected the yield and quality of peanuts and caused a large-scale reduction in peanut production. [0003] Underground pests refer to omnivorous pests that harm the main stems, roots, and seeds of plants near the soil surface at a certain stage in their life history, causing damage to plant roots, affecting plants to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/56A01H5/00
CPCC12N9/2497C12N15/8286C12Y302/02016
Inventor 耿丽丽张杰冯艳芳束长龙彭琦宋福平梁影屏
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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