A microfluidic experimental device and method for optimal control of cell culture conditions

Abstract

The invention discloses a micro-fluidic experimental device and method for cell culture condition optical control and belongs to the micro-fluidic and biomedicine analysis technique.A micro-fluidic chip is provided with an inner layer and an outer layer outside a culture room in the diameter direction, the inner layer and the outer layer are isolated from each other in a sealed mode, a dry powder solution sample introduction pool, a serum solution sample introduction pool, a cancer cell sample introduction pool and a wastewater pool are arranged in the inner layer, and the outer layer is a circle of out-layer water channel; an electromagnet and a direct-current power supply connected with the electromagnet are arranged below the micro-fluidic chip, three solution injection syringes are arranged above the micro-fluidic chip, a plurality of dilution branches are connected between the outer-layer water channel and sample introduction channels close to the end of the sample introduction pool in parallel, a piezoelectric micro valve is installed on each dilution branch, and each piezoelectric disc is connected with an electromagnetic relay in series and then connected with the direct-current power supply.The number of water channels is controlled by means of the multiple piezoelectric micro valves, the purpose of culture solution dilution is realized, and dilution of solutions with various concentrations can be achieved.

Description

technical field [0001] The invention relates to the field of microfluidic technology and the field of cell culture technology in biomedical analysis, in particular to a cell culture condition optimization control device based on a microfluidic chip. Background technique [0002] Cell culture technology refers to the growth of cells under in vitro conditions in which the cells no longer form tissues (animals). A culture is a single cell or a population of cells. Cells must live in an artificial environment during culture. Due to environmental changes, cell movement or the influence of some other factors, the culture time is prolonged, and the passage leads to a single type of cells. The application of cell culture technology is to directly observe the morphological structure and life activities of living cells. It is used in the research of various disciplines such as cytology, genetics, immunology, experimental medicine and oncology. Direct observation of cell changes can f...

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Problems solved by technology

However, the device lacks the ratio processing link of the culture solution, the concentration of the culture solution required by the cells cannot be determined, the concentration ratio of the culture solution cannot be recorded, and it is impossible to compare whether the culture solution is suitable for the survival of the cells

In addition, in the cultivation process, the solution cannot be reused, resulting in a certain degree of waste, thereby increasing the cost

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