Human Lp-PLA2 biotin-streptavidin fluorescence immunochromatographic assay card and preparation method thereof

Abstract

The invention discloses a human Lp-PLA2 biotin-streptavidin fluorescence immunochromatographic assay card and a preparation method thereof and aims at providing an assay card which is supportive of quick combining, free of outside interference during reaction and high in specificity and stability and a preparation method thereof. The technical scheme includes that assay test paper comprises a substrate, a sample pad, a mark pad, a coated film and an absorption pad are connected on the substrate. The assay card is characterized in that an anti-Lp-PLA2 monoclonal antibody 1 of fluorescent microspheres and a biotin anti-Lp-PLA2 monoclonal antibody 2 are coupled on the mark, a streptavidin assay line T and a goat-anti-mouse polyclonal antibody quality control line C are parallelly arranged on the coated film. The invention belongs to the field of fluorescence immunochromatographic assay.

Description

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AI Technical Summary

Benefits of technology

This patented innovation improves upon previous methods for detecting certain molecules like proteins or DNA sequences through their ability to attach them onto special surfaces called streptoavidin particles. These techniques have been developed overall, making these technologies more effective at identifying small quantities of target materials such as nucleic acids.

Problems solved by technology

Technological Problem: Current Cardiac Diseasemic Threatening (CVD), including Acute Coronary Artery Stenosis(ACS)/ Clinically Importated Statement (CAI). There currently exist various therapies such as antiplatelet agents or calcium blocker drugs, while none provide satisfactory results due to their limitations. Therefore, research efforts aim at developing novel biotherapy targets and diagnosing techniques capable of early diagnosis without requiring costly procedures like histopathology analysis.

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