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Cyanobacteria culture medium

A culture medium and cyanobacteria technology, applied in the field of ancient oil and gas exploration experiments, can solve the problems of cyanobacteria growth and destruction, and achieve the effect of increasing the amount of oil

Inactive Publication Date: 2016-04-13
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, domestic research on the growth of cyanobacteria under radioactive conditions is in a blank stage. Most of them are studying the influence of radiation conditions on the development of cyanobacteria. The conclusion is that radiation has a destructive effect on the growth of cyanobacteria.

Method used

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  • Cyanobacteria culture medium
  • Cyanobacteria culture medium
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] In this example, a standard 100mL Bg11 medium was used, and three uranium octaoxide at a mass concentration of 3ppm was added to the medium;

[0047] Microcystis aeruginosa species were inoculated into the medium for dilution, and the medium with the mass concentration of U3O8 was set into 3 parallel groups.

Embodiment 2

[0049] In this example, a standard 100mL Bg11 medium was used, and uranium octaoxide at a mass concentration of 15ppm was added to the medium;

[0050] Microcystis aeruginosa species were inoculated into the medium for dilution, and the medium with the mass concentration of U3O8 was set into 3 parallel groups.

Embodiment 3

[0052] In this example, a standard 100 mL Bg11 medium was used, and uranium octaoxide at a mass concentration of 75 ppm was added to the medium;

[0053] Microcystis aeruginosa species were inoculated into the medium for dilution, and the medium with the mass concentration of U3O8 was set into 3 parallel groups.

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Abstract

The invention provides a cyanobacteria culture medium. The cyanobacteria culture medium is prepared from a Bg11 culture medium and triuranium octoxide, wherein the mass concentration of the triuranium octoxide is 1 ppm-100 ppm when the total mass of the Bg11 culture medium is taken as the criterion. The invention further provides a method for verifying the influence of radioactive substances on cyanobacteria culture. The method comprises the steps that the triuranium octoxide is added into the Bg11 culture medium, and a test for the growth speed and organic matter enrichment is performed on obtained cyanobacteria, wherein the dosage of the triuranium octoxide is 1 ppm-100 ppm when the total mass of the Bg11 culture medium is taken as the criterion. The cyanobacteria culture medium can enable the cyanobacteria to be prosperous and enable cyanobacteria organic matter to be extensively enriched.

Description

technical field [0001] The invention relates to a culture medium, in particular to a cyanobacteria culture medium, and belongs to the field of ancient oil and gas exploration experiment technology. Background technique [0002] As the main source material of Meso-Neoproterozoic source rocks in North China, cyanobacteria have a great influence on the generation of Meso-Neoproterozoic oil and gas in North China due to their developmental potential and scale. Exploring the geological factors that affect the development of cyanobacteria is of great significance to the developmental potential and scale assessment of Meso-Neoproterozoic cyanobacteria. The Chernobyl and Fukushima nuclear accident pollution areas have caused the abnormal growth of organisms in the adjacent sea areas. The normal natural gamma ray curve (including radioactivity) of Well XX in the Ordos Basin has a good agreement with the organic carbon curve, and the concentrated section of volcanic material (high rad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 马奎王铜山石书缘赵伟波
Owner PETROCHINA CO LTD
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