Use of albiflorin in preparation of laryngeal cancer resistant medicines

A technology of paeonifloride glycosides and uses, applied in the field of paeonifloride glycosides in the preparation of anti-tumor drugs, which can solve the problems of limited quality control, difficulty in mass screening of anti-tumor active ingredients, and complex composition of compounds

Inactive Publication Date: 2015-06-17
JINGPENGHUI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, due to the complex composition of the compounds in most traditional Chinese medicines, the difficulty of separation and purification, the limited quality control and the unclear efficacy and mechanism of action, it is very difficult to screen a large number of anti-tumor active ingredients, so the research on the active ingredients of anti-tumor traditional Chinese medicine is widespread. There are many deficiencies and limitations

Method used

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  • Use of albiflorin in preparation of laryngeal cancer resistant medicines
  • Use of albiflorin in preparation of laryngeal cancer resistant medicines
  • Use of albiflorin in preparation of laryngeal cancer resistant medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 The effect of paeoniflorin on tumor cell proliferation

[0031] Experimental scheme: Thiazolyl blue (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide, MTT) experiment was used, and paeoniflorin was used to treat rhabdomyosarcoma cells A204, Nasopharyngeal cancer cells CNE-2Z, intestinal cancer cells CT26, intestinal cancer cells HT29, neuroblastoma cells SY5Y, laryngeal cancer cells Hep2, pancreatic cancer cells PANC1, cervical cancer cells Hela, breast cancer cells MDA231, breast cancer cells T47D, Breast cancer cells MCF7, skin squamous cell carcinoma cells A431, kidney cancer Wilms cells G401, and prostate cancer cells DU145 for about 24 hours; after MTT staining for 4 hours, dimethyl sulfoxide (DMSO) was dissolved, and the absorbance value was measured at 570 nm by a microplate reader .

[0032] Experimental results: Paeoniflorin was found to be effective in pancreatic cancer cells PANC1, intestinal cancer cells CT26, intestinal cancer cells HT2...

Embodiment 2

[0036] Example 2 The effect of paeoniflorin on tumor cell migration (Transwell assay and Scratch Analysis assay)

[0037] Experimental protocol: 1) Using cell migration assay (Transwell) assay, A549, LLC, Hela, SY5Y, CT26, T47D, MCF7, MDA231, A204, G401, CNE-2Z, DU145, Hep2, PANC1 and SW13 tumor cells were dosed After 24 hours of paeoniflorin (a safe dose of 20 μg / ml was selected), the cells were fixed in anhydrous ice methanol for 20 minutes, stained with crystal violet for 15 minutes, and photographed under a 100-fold light microscope to detect the number of cells migrated.

[0038] 2) Using cell migration assay (Scratch Analysis), to Hela, LLC, A549, SY5Y, CT26, MCF7, MDA231 and T47D cells plus paeoniflorin for 6 hours and 24 hours (select a safe dose of 20μg / ml), 40 times Light microscope photographs were taken to detect cell spacing.

[0039] Experimental results: 1) Transwell experiments showed that paeoniflorin (AL) can significantly inhibit lung cancer cells A549, lun...

Embodiment 3

[0042] Example 3 Comparison of the effect of paeoniflorin and total glucosides in paeony on tumor inhibition

[0043] 1. Comparison of the effects of paeoniflorin and total glucosides of paeony on tumor cell proliferation (MTT)

[0044] Experimental scheme: Thiazolyl blue (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide, MTT) experiment was used, and paeoniflorin and total glucosides of paeony were treated respectively A204, CNE-2Z, CT26, Hep2 and PANC1 tumor cells were stained for 24 hours, then MTT was stained for 4 hours, dimethyl sulfoxide (DMSO) was dissolved, and the absorbance value was measured at 570 nm by a microplate reader.

[0045] Experimental results: It was found that paeoniflorin and total glucosides of paeony have inhibitory effects on the proliferation of rhabdomyosarcoma cells A204, nasopharyngeal cancer cells CNE-2Z, intestinal cancer cells CT26, laryngeal cancer cells Hep2 and pancreatic cancer cells PANC1. IC of lactones on these tumors 50 (...

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Abstract

An application of a paeoniflorin compound, or a pharmaceutically acceptable salt, solvate, polymorph, enantiomer, or racemic mixture thereof in preparation of drugs for preventing and / or treating tumors. Preferably, the paeoniflorin compound is selected from albiflorin and / or paeoniflorin. The tumors are solid tumors.

Description

[0001] This application is a divisional application of a Chinese invention patent application with the application number of 201310180327.7, the application date is May 15, 2013, and the invention name is "use of paeoniflorin compounds in the preparation of antitumor drugs". technical field [0002] The invention belongs to the field of antitumor drugs, and relates to the use of paeoniflorin compounds in the preparation of antitumor drugs, in particular to the use of paeoniflorin in the preparation of antitumor drugs. Background technique [0003] Tumor is a new organism formed by abnormal clonal proliferation of cells under the action of various carcinogenic factors. Tumor cells are different from normal cells in external morphology, metabolism and function, and most of them show continuous proliferation. According to effective statistics, tumor is the first of the three major factors that threaten human life and health in the world, so the research and development of anti-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7048A61P35/00A61P11/04
CPCA61K31/7048A61P11/04A61P35/00
Inventor 李梢
Owner JINGPENGHUI PHARMA
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