Inverted laser scanning confocal microscope animal and plant living body observation device and method

Abstract

The invention discloses an inverted laser scanning confocal microscope animal and plant living body observation device, belonging to the technical field of microstructures and structure observation. The traditional cell biology experiment is performed on animal and plant living bodies, and by using the device disclosed by the invention for observing the interior of local organs and tissues of the animal and plant living bodies, a high-precision microscopic image can be acquired, and the result is reliable. Meanwhile, multiple indexes are acquired, a dynamic change effect of animal and plant tissue cells and tissue levels is achieved, a basis is provided for further scientific research, change of the treated organism is traced, and pertinence measures can be taken in the accurate time. The problems that the detection lags behind drug actions and the detection result is not intuitive are solved in the animal pharmacology aspect, the action time and action effect of the drugs in animal bodies can be detected in real time, and the device and method have intuitive guiding effects on the drug dose and drug administration time.

Description

technical field [0001] The invention belongs to the technical field of microstructure and structure observation, and in particular relates to an inverted laser confocal microscope living animal and plant observation device and method. Background technique [0002] The laser scanning confocal microscope is an epoch-making high-tech product developed in the 1980s. It is based on the fluorescence microscope imaging with a laser scanning device, and uses a computer for image processing. The efficiency has been increased by 30% to 40%, and ultraviolet or visible light is used to excite fluorescent probes to obtain fluorescent images of the fine structures inside cells or tissues. Laser Scanning Confocal Microscopy to Observe Ca 2+ , pH value, membrane potential and other physiological signals and changes in cell morphology have become a new generation of powerful research tools in the fields of morphology, molecular biology, neuroscience, pharmacology, and genetics. [0003] Th...

AI Technical Summary

Problems solved by technology

[0005] However, confocal microscopes have high requirements for samples, and conventional observations can only be performed on glass slides and petri dishes, and high-magnification live cell observation can only be completed in special petri dishes, and cannot be used for live imaging of animals and plants. The functions of multi-channel fluorescence simultaneous detection and multi-color fluorescence video recording are limited in the field of live observation

Recently, the survival and transfer experiments of stem cells transfected with GFP can only be tested by killing the animals and making slides. The samples must be fixed a

Images