Transgenic breeding method of sweet wormwood

Abstract

The invention provides a transgenic breeding method of sweet wormwood. The transgenic breeding method of sweet wormwood comprises the following steps: taking immature sweet wormwood inflorescence as an explant, infecting the explant by using an agrobacterium solution with exogenous genes, co-culturing the explant for 2-3 days, putting the explant on a selective-pressure culture medium to induce and generate a callus tissue, further inducing the callus tissue into a regeneration plant, then carrying out molecular biological detection on a reporter gene, and identifying a transgenic plant in the regeneration plant. According to the transgenic breeding method of sweet wormwood, the immature sweet wormwood inflorescence is used as the explants and is co-cultured with the agrobacterium EHA105 strains, so that a transformation rate is high; the highest transformation rate is 17.2% and is higher than that of the common leaf explant transgenic rate in the current sweet wormwood transgenosis by 8.59%. A novel transgenic system is provided for the genetic transformation of the sweet wormwood; meanwhile, the reference is provided for other plants difficult in agrobacterium-mediated transformation.

Description

technical field [0001] The invention relates to gene breeding, in particular to a method for transgenic breeding of Artemisia annua. Background technique [0002] Malaria is a serious life-threatening disease, with approximately one million deaths worldwide and approximately 3.5-5 million new cases each year (Graham et al.2010; Graham et al.2010; Milhous and Weina 2010 ). [0003] For a long time, Artemisia annua is a special medicinal plant used to treat malaria. Artemisinin, a sesquiterpene lactone endoperoxide, is currently recognized as the most effective compound against multidrug-resistant Plasmodium falciparum (Weina 2008; Li et al. 2006). Artemisinin also has the ability to treat certain cancers and other viral and parasitic diseases (Efferth 2007; Efferth et al. 2008; Weathers et al. 2011). However, due to the low artemisinin content in A. annua plants ( 0.01%-0.8% of dry weight). The use of artemisinin-based drugs has been limited, especially in developing coun...

AI Technical Summary

Problems solved by technology

Since then, researchers have done a lot of work on transgenic methods of Artemisia annua, with little success

Many studies have proved that transgenic Artemisia annua is very difficult because it is laborious, time-consuming, and more importantly, the transformation efficiency is very low (less than 10%) (Liu et al. 2011)

Images