Micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects

Abstract

The invention belongs to the field of micro-fluidic chips, and discloses a micro-fluidic chip device for separating bacteria by using laminar flow and chemotaxis effects. The device comprises two parts namely a micro-fluidic chip for separating the bacteria and an inducing reagent concentration control system, wherein the micro-fluidic chip is an approximately 'X'-shaped PDMS-glass chip which is integrated with an inducing reagent inlet, a bacterium inlet, a bacterium separation channel, a bacterium collection outlet and a waste liquid outlet; and the inducing reagent concentration control system consists of a plurality of groups of injection pumps and injectors, parameters such as flow rates and the like can be controlled by virtue of the injection pumps, and thus an inducing reagent of which the concentration dynamically changes with time can be obtained. According to the micro-fluidic chip device disclosed by the invention, operations of separating interested bacteria from a bacterial mixed liquid can be achieved by mainly using the characteristics that the bacteria swim with tendency to high attractant concentration and low repellent concentration directions under the action of laminar flows and chemical inducing reagents. The micro-fluidic chip device disclosed by the invention can be applied to research and analysis of the fields of food safety, water quality monitoring, pharmaceutical engineering, clinical medicines and the like.

Description

technical field [0001] The invention belongs to the field of biological microfluidic chips, in particular to a microfluidic chip device for separating bacteria by using laminar flow and chemotaxis. Background technique [0002] Bacteria isolation refers to the process of isolating bacteria of interest from a mixture of bacteria and cells, or a mixture of various bacteria. It is of great significance in the food industry, water quality testing, bioengineering, pharmaceutical industry and clinical medicine. [0003] The traditional bacterial isolation is carried out in agar medium, the culture conditions are harsh, time-consuming, and the isolation and culture process is easily contaminated by miscellaneous bacteria, so the bacteria isolation and culture based on this method often cannot obtain ideal results. In recent years, the development of microfluidic technology has made the rapid isolation of bacteria possible. Using the swimming and rolling characteristics of bacteria...

AI Technical Summary

Problems solved by technology

Using the swimming and rolling characteristics of bacteria in laminar flow, people have established a variety of microfluidic chips for separating bacteria, but the separation efficiency of these bacteria separation chips is still low

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