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A kind of breeding method of all-fragrant soft rice three-line hybrid rice

A technology of three-line hybrid rice and fragrant type, which is applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., and can solve problems such as hardening, poor cooking, and bad taste

Active Publication Date: 2016-01-13
文山壮族苗族自治州农业科学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some defects in these fragrant hybrid rice breeding methods: first, the quality of the paired parents is not good, such as high amylose content, not soft rice type, although the paired breeding can obtain full-fragrant hybrid rice varieties or natural ginseng type However, due to the high content of amylose, the rice will regenerate after cold, harden and taste bad; the second is to match the parents, that is, the quality of the sterile line and the restorer line are inconsistent, such as the content of amylose is inconsistent , leading to hybrid rice (F 2 Genetic segregation occurs in the quality and shape of the generation seed) to form mixed rice with different softness and hardness, which is of poor quality and is not easy to cook; third, although both parents of the mating group have fragrance, but because the genes that control fragrance are often unequal, hybrid mating Most of the hybrid rice varieties obtained are not full-flavored varieties
However, compared with hybrid rice varieties, due to the lack of richer genetic basis of hybrid rice varieties, the recombination of more good genes, and the expansion of genetic differences, the yield has always been reduced by more than 20% compared with hybrid rice varieties, resulting in indica in rice production. High-yield hybrid rice, but poor quality, while high-quality aromatic soft rice conventional rice is good in quality, but the situation is low, which cannot meet the increasing requirements of domestic and foreign markets for rice quality, let alone adapt to the national food security strategy and the international market. competing needs of

Method used

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  • A kind of breeding method of all-fragrant soft rice three-line hybrid rice
  • A kind of breeding method of all-fragrant soft rice three-line hybrid rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 Screening of aromatic soft rice resources

[0041] The flow chart of the present invention carrying out the high-quality hybrid rice selection of full-flavor type soft rice with conventional breeding is as follows figure 1 As shown, the mark "can be the parents of each other" in the place of "fragrant soft rice variety I × maintainer line II" means that the fragrant soft rice variety I can be used as the female parent, and the maintainer line II can be used as the male parent For hybridization, the maintainer line II can also be used as the female parent, and the fragrant soft rice rice maintainer variety I can be used as the male parent, and so on for the "aroma soft rice restorer variety VI × restorer line VII".

[0042] If in combination with biotechnology to carry out the high-quality hybrid rice breeding of whole-fragrant soft rice of the present invention, it is: Utilize conventional biotechnological means to control the gene a of fragrance and the ge...

Embodiment 2

[0050] Example 2 Creation of Wenxiang 28B and CMS Wenxiang 28A, a maintainer line of fragrant soft rice

[0051] In this example, Diantun 502 is used as the aroma and soft rice gene donor of the present invention——aroma type soft rice rice maintenance variety I, and F of Jin 23B×Longtepu B 1 The generation is used as the material for maintaining the gene donor—the maintainer line II, and the sterile line Zhenshan 97A is used as the sterile line IV, according to figure 1 Breeding of fragrant soft rice maintainer line III and fragrant soft rice high-quality male sterile line V, specifically:

[0052] In the autumn of 1998, in the network room of Wenshan Academy of Agricultural Sciences, the F of Jin 23B × Longtepu B 1 Diantun 502 was used as the female parent to carry out hybridization, and then according to the breeding objectives of rice grains with aroma, soft rice, long grain, and transparency, the pedigree method and the method of increasing selection pressure were used to...

Embodiment 3

[0059] Example 3 Creation of Wenhui 287, a high-quality restorative line of fragrant soft rice

[0060] In this embodiment, Hongyou 1 is used as the aroma and soft rice gene donor variety of the present invention—fragrant soft rice rice VI, and Feihui 6 is used as the strong restorer gene donor material—restorer line VII. figure 1 Breeding high-quality restorer line VIII of fragrant soft rice, specifically:

[0061] In 2001, in the network room of Wenshan Academy of Agricultural Sciences, the fragrant soft rice rice Hongyou No. 1 was used as the female parent, and Feihui No. 6, an excellent restorer line with high combining ability, strong resilience and wide adaptability, was used as the male parent. After hybridization, according to the breeding goals of rice grains having aroma, soft rice, long grain, and transparency, the pedigree method and the method of increasing selection pressure are used to identify the aroma of brown rice by chewing method and other selection scheme...

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Abstract

The invention discloses a full-aroma soft-rice high-quality hybrid paddy rice breeding method. The method comprises the specific steps that: aromatic soft-rice paddy rice maintainer variety I with a genotype of aabb and aromatic soft-rice paddy rice restorer variety VI with a genotype of aabb are screened; aroma-controlling gene a and low grain straight-chain starch content gene b in the aromatic soft-rice paddy rice maintainer variety I and the aromatic soft-rice paddy rice restorer variety VI are respectively introduced into a maintainer line and a restorer line; an obtained maintainer line is transferred to a sterile line, such that an aromatic soft-rice maintainer line III, an aromatic soft-rice high-quality restorer line VIII, and an aromatic soft-rice high-quality male sterile line V with genotype of aabb are obtained; the aromatic soft-rice high-quality male sterile line V is adopted as a female parent, and is matched with the aromatic soft-rice high-quality restorer line VIII, such that the full-aroma soft-rice high-quality hybrid paddy rice Z with the genotype of aabb is obtained. The full-aroma soft-rice high-quality hybrid paddy rice Z has high quality, and is aromatic in every single grain. The rice is soft rice, such that cooked rice is prevented from returning underdone after cooling.

Description

technical field [0001] The invention relates to a hybrid rice breeding technology, in particular to a method for selecting a whole-fragrant soft rice three-line hybrid rice. Background technique [0002] The successful invention and application of indica hybrid rice breeding methods in my country has created huge economic and social benefits, and has been highly appraised at home and abroad. However, hybrid rice is a segregated F 2 Generation seeds, the quality traits are between the parents, the combination of high-quality preparation of both parents, especially the allelism of high-quality trait genes such as fragrance genes, can fundamentally prevent F 2 Genetic segregation of rice grains, so that the internal quality of rice grains does not separate or the separation is controlled to a minimum. Since the physical and chemical indicators of the parents of high-quality three-line hybrid rice in my country have all reached the national excellent level 3 or above, it is di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02
Inventor 李存龙罗龙杨芬陶永宏韦永贵申仕安董卓娅
Owner 文山壮族苗族自治州农业科学院
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